Caitlin Lanni

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Rabbit peritoneal neutrophils secrete phospholipase A2 activity in response to f-Met-Leu-Phe (FMLP). The secretion of phospholipase A2, like that of the known granule enzymes, requires cytochalasin B and is enhanced by extracellular Ca2+, and the time course of the release is rapid, being completed in less than a minute. The concentration dependence of the(More)
Highly purified Ca2+-dependent phospholipases A2 that were isolated from human platelets, rabbit alveolar macrophages and peritoneal polymorphonuclear leucocytes and were active in the neutral-to-alkaline pH range were inhibited 50% by 75 microM-indomethacin in the presence of 5.0 mM added Ca2+. Sodium meclofenamate and sodium flufenamate were also(More)
Milrinone (Corotrope) increased cyclic AMP levels in dog guinea pig and rat cardiac muscle. A correlation between the increase in contractile force and cyclic AMP levels in dog ventricular trabeculae was obtained when measurements were made 60-70 s after the addition of milrinone. When cyclic AMP levels were determined at the time of maximal contractile(More)
The lipoxygenase and/or cyclooxygenase inhibitors nordihydroguaiaretic acid (NDGA), 4,8,11,14-eicosatetraynoic acid (ETYA) and the bioflavonoid, quercetin, also inhibit phospholipase A2 (phosphatidase 2-acyl hydrolase; EC 3.1.1.4) activity of neutrophil acid extracts and sonicates. The IC50 are 13 microM for NDGA, 22 microM for ETYA, and 100 microM for(More)
The localization and partial purification of a Ca2+-dependent, membrane associated phospholipase A2 (phosphatide 2-acylhydrolase, EC 3.1.1.4) from BCG-induced rabbit alveolar macrophages is described. Phospholipase A activity was determined using autoclaved Escherichia coli, the phospholipids of which were labelled in the 2-acyl position with [1-14C]oleate.(More)
Iron oxide cores of 35 nm are coated with gold nanoparticles so that individual particle motion can be tracked in real time through the plasmonic response using dark field optical microscopy. Although Brownian and viscous drag forces are pronounced for nanoparticles, we show that magnetic manipulation is possible using large magnetic field gradients. The(More)
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