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It has been reported that the propagation of African swine fever virus (ASFV) in cell culture generates viral subpopulations differing in protein p54 (C. Alcaraz, A. Brun, F. Ruiz-Gonzalvo, and J. M. Escribano, Virus Res. 23:173-182, 1992). A recombinant bacteriophage expressing a 328-bp fragment of the p54 gene was selected in a lambda phage expression(More)
We have identified and characterized a 30-kDa phosphoprotein (p30) of African Swine Fever Virus (ASFV) that is synthesized, membrane localized, and released into the culture medium at early times after infection. Sequence analysis of the p30 open reading frame predicts a highly antigenic protein with putative phosphorylation, glycosylation, and membrane(More)
A Western blot technique using a recombinant protein has been developed to confirm positive results obtained in African swine fever (ASF)-specific antibody detection by ELISA. The new confirmatory Western blot is based on the use of protein p54, one of the most antigenic ASF virus structural proteins, expressed in Escherichia coli fused to the N-terminus of(More)
We have detected 86 African swine fever (ASF) virus-induced proteins in infected pig macrophages by two-dimensional electrophoresis. No differences among protein patterns of wild-type viruses could be observed by this methodology. However, during cell culture adaptation and propagation we have characterized changes in the molecular weight of the ASF virus(More)
The present work describes a simple dot immunobinding assay (DIA) for African swine fever virus (ASFV) antibody detection that can be used under field conditions. The assay uses nitrocellulose strips dotted with a cytoplasmic soluble antigen (CS-P) of ASFV. The nitrocellulose strips are adhered to a plastic handle. The test serum samples react with the(More)
The African swine fever virus-induced proteins on plasma membranes of infected cells have been studied by two different procedures, iodination and incubation of infected cells labeled with [35S]methionine with a specific antiserum, obtained from pigs immunized with a monkey stable cell-adapted African swine fever virus. The combined use of both procedures(More)
African swine fever (ASF) virus-induced plasma membrane proteins may contribute to the protective immune response against the disease since they can be involved in the antibody-mediated lysis of infected cells. In this study we describe the regulation of ASF virus-induced plasma membrane protein expression and its antibody induction in pigs after viral(More)
Suspended load dynamics were analyzed for the period 2007-2009 in a semi-meandering cross-section under different hydrological conditions. Samples were collected at four different points of the cross-section. During "low discharges" (≤ 600 m³/s) suspended load samples were collected at-a-monthly basis, whereas at "high discharges" (> 600 m³/s) sampling was(More)
In this study, we have investigated the characteristics of secreted IgA and other classes of Ig induced after vaccination of sows with transmissible gastroenteritis virus (TGEV) or the antigenically related porcine respiratory coronavirus (PRCV). Both viruses induced the secretion of neutralizing antibodies of different classes in the sows' milk, but these(More)
The southeast of the Iberian Peninsula has traditionally suffered a great extraction of their mineral resources. Mining activity was originated more than 2500 years ago. As its consequence while operating, and after its decline and end, high amounts of wastes have remained within mining areas. These materials, accumulated on the surface, are strongly(More)