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In studies with pyridoxine and other B(6) compounds in blood, the active forms pyridoxal and pyridoxal phosphate were measured by differential assays using Lactobacillus casei. Red cell uptake of tritiated pyridoxine was also measured. A new metabolic pathway for conversion of pyridoxine to active forms was demonstrated in red cells. In vivo studies in(More)
A new method has been developed for the assay of serum pyridoxal using L. casei. Bound pyridoxal phosphate in serum is converted by acid hydrolysis to pyridoxal for which this organism is specific. This method proved to be considerably more sensitive than other methods so far reported in the literature. Serum pyridoxal concentrations were measured in 151(More)
This work was presented in part at the 8th International Congress of Haematology, Munich 1970. Received for publication 30 November 1970 and in revised form 25 February 1971. Pyridoxamine was converted to active forms in red cells less efficiently. Pyridoxal entered red cells rapidly, equilibrating between plasma and cells within 1 min in the same ratio as(More)
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