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Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
TLDR
There continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes, so it is important to update guidelines for monitoring autophagic activity in different organisms.
View on Taylor & Francis
Salmonella effectors within a single pathogenicity island are differentially expressed and translocated by separate type III secretion systems
TLDR
A functional and regulatory cross‐talk between three chromosomal PAIs, SPI‐1, SPI-2 and SPI‐5, has significant implications for the evolution and role of PAIs in bacterial pathogenesis.
View on Wiley
A conserved structural motif mediates formation of the periplasmic rings in the type III secretion system
TLDR
Using an integrated structural and experimental approach, ring models for the periplasmic domain of EscC are generated and placed in the context of the assembled T3SS, providing evidence for direct interaction between the outer membrane and inner membrane ring components and an unprecedented span of the outer membranes secretin.
View on Nature
Accurate prediction of protein structures and interactions using a 3-track neural network
TLDR
A three-track network produces structure predictions with accuracies approaching those of DeepMind in CASP14, enables the rapid solution of challenging X-ray crystallography and cryo-EM structure modeling problems, and provides insights into the functions of proteins of currently unknown structure.
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Structural characterization of the molecular platform for type III secretion system assembly
TLDR
Crystal packing analysis and molecular modelling indicate that EscJ could form a large 24-subunit ‘ring’ superstructure with extensive grooves, ridges and electrostatic features, and it is proposed that the YscJ/PrgK protein family functions as an essential molecular platform for TTSS assembly.
View on Nature
A Structure-Based Mechanism for Vesicle Capture by the Multisubunit Tethering Complex Dsl1
View on Elsevier
Structure of the human mTOR complex I and its implications for rapamycin inhibition.
View on PubMed
Structural characterization of a type III secretion system filament protein in complex with its chaperone
TLDR
It is shown that EspA alone is sufficient to form filamentous structures in the absence of other pathogenicity island–encoded proteins, and it is demonstrated that CesA traps EspA in a monomeric state and inhibits its polymerization.
View on Nature
CesT is a multi‐effector chaperone and recruitment factor required for the efficient type III secretion of both LEE‐ and non‐LEE‐encoded effectors of enteropathogenic Escherichia coli
TLDR
In vivo co‐immunoprecipitation studies using CesT‐FLAG containing EPEC lysates demonstrated that CesT interacts with Tir and EscN, consistent with the notion of CesT recruiting Tir to the T3SS, and functionally redefine the role of Ces T in multiple type III effector interactions.
View on Wiley
Atg29 phosphorylation regulates coordination of the Atg17-Atg31-Atg29 complex with the Atg11 scaffold during autophagy initiation
TLDR
It is demonstrated that Atg29 is a phosphorylated protein and that this modification is critical to its function; alanine substitution at the phosphorylation sites blocks its interaction with the scaffold protein Atg11 and its ability to facilitate assembly of the PAS.
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