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Natural dehydration regimes as a prerequisite for the successful induction of anhydrobiosis in the nematode Rotylenchulus reniformis.
Induction of anhydrobiosis in the nematode Rotylenchulus reniformis (Linford & Oliveira) was studied using direct exposure to elevated relative humidities and conditions resembling natural… Expand
Preservation of freeze-dried liposomes by trehalose.
- L. Crowe, J. Crowe, A. Rudolph, C. Womersley, L. Appel
- Chemistry, Medicine
- Archives of biochemistry and biophysics
- 1 October 1985
One of the practical difficulties with the frequently proposed use of liposomes for delivery of water-soluble substances to cells in whole organisms is that liposomes are relatively unstable during… Expand
Critical aspects of fluorescent age-pigment methodologies: Modification for accurate analysis and age assessments in aquatic organisms
Fluorescent age-pigment (FAP) quantification has recently been proposed as a means of estimating age in aquatic vertebrates and invertebrates. However, various aspects related to currently adopted… Expand
Effects of carbohydrates on membrane stability at low water activities.
- L. Crowe, R. Mouradian, J. Crowe, S. A. Jackson, C. Womersley
- Chemistry, Medicine
- Biochimica et biophysica acta
- 11 January 1984
The relative effectiveness of a variety of carbohydrates in preserving the structural and functional integrity of membranes at low water activities was studied, using Ca-transporting microsomes from… Expand
Biochemical and physiological aspects of anhydrobiosis
- C. Womersley
1. 1. The biochemical and physiological mechanisms which are involved in anhydrobiotic survival have been reviewed. 2. 2. The physical state of water within hydrated and dehydrated organisms is… Expand
Prevention of fusion and leakage in freeze-dried liposomes by carbohydrates
Abstract The ability of various carbohydrates to prevent leakage of a small, water-soluble marker from unilamellar liposomes during freeze-drying has been investigated and compared with the ability… Expand
Separation of tricarboxylic acid cycle acids and other related organic acids in insect haemolymph by high-performance liquid chromatography
Anhydrobiosis in nematodes—I. The role of glycerol myo-inositol and trehalose during desiccation
Abstract 1. 1. The concentrations of free glycerol, inositol and trehalose in five species of nematodes were determined. Analyses of total inositol content were also made. 2. 2. Significant… Expand
Inhibition of dehydration-induced fusion between liposomal membranes by carbohydrates as measured by fluorescence energy transfer.
The relative abilities of a number of naturally occurring carbohydrates to inhibit dehydration-induced fusion between palmitoyloleoylphosphatidylcholine:phosphatidylserine (85:15) large unilamellar… Expand