C. Wolk

Publications175
h-index58
Citations11,835
Highly Influential Citations592
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Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120.
TLDR
Only 37% of the Anabaena genes showed significant sequence similarity to those of Synechocystis, indicating a high degree of divergence of the gene information between the two cyanobacterial strains.
A versatile class of positive-selection vectors based on the nonviability of palindrome-containing plasmids that allows cloning into long polylinkers.
Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena
TLDR
The spatially patterned differentiation of hetero‐cysts in the filamentous cyanobacterium Anabaena requires a functional hetR gene, indicating that hetG is autoregulatory and full induction of a heterocyst structural gene, hepA, also requires afunctional het R locus.
Conjugal transfer of DNA to cyanobacteria.
Use of a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion sequences
TLDR
Use of the sacB gene provides a simple, effective, positive selection for double recombinants in Anabaena sp.
Heterocyst Metabolism and Development
TLDR
An autoregulated gene, hetR, that is activated shortly after nitrogen-stepdown is critical for the differentiation of heterocysts, and an evolutionary and biochemical relationship between the processes leading to the formation ofheterocysts and akinetes is suggested.
Use of a transposon with luciferase as a reporter to identify environmentally responsive genes in a cyanobacterium.
TLDR
A derivative of transposon Tn5 was used to generate transcriptional fusions of promoterless bacterial luciferase genes, luxAB, to the Anabaena genome and permitted high-resolution mapping of its position and orientation in the genome and facile cloning of contiguous genomic DNA.
[83] Conjugal transfer of DNA to cyanobacteria
Reduction of conjugal transfer efficiency by three restriction activities of Anabaena sp. strain PCC 7120
The efficiency of conjugal transfer of plasmids from Escherichia coli to the cyanobacterium Anabaena sp. strain PCC 7120 was quantitated as a function of the number of restriction sites for the…
Analysis of a Het- mutation in Anabaena sp. strain PCC 7120 implicates a secondary metabolite in the regulation of heterocyst spacing
Transposon-generated mutant N10 of Anabaena sp. strain PCC 7120 has a Het- phenotype (A. Ernst, T. Black, Y. Cai, J.-M. Panoff, D. N. Tiwari, and C. P. Wolk, J. Bacteriol. 174:6025-6032, 1992).…
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