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Fructose-1,6-Diphosphate-Dependent Lactate Dehydrogenase from a Cariogenic Streptococcus: Purification and Regulatory Properties
Results suggested that there may be two types of pyruvate binding sites on the LDH from S. mutans, which appeared to be at least two binding sites for the activator which interacted in a cooperative manner.
Purification and Properties of a Fructose-1,6-Diphosphate-Activated Lactate Dehydrogenase from Streptococcus faecalis
Evidence was found that FDP lowered significantly the apparent K(m) for both pyruvate and reduced nicotinamide adenine dinucleotide, and the enzyme, which was quite heat stable in the absence of any of the reactants, was rendered heat labile by FDP.
Mannitol transport in Streptococcus mutans
A hexitol-inducible, phosphoenolpyruvate-dependent phosphotransferase system was demonstrated in Streptococcus mutans and divalent cations Mn2+ and Ca2+ partially replaced Mg2+, while Zn2+ was found to be highly inhibitory.
Characterization of a phosphoenolpyruvate-dependent sucrose phosphotransferase system in Streptococcus mutans
A phosphoenolpyruvate-dependent sucrose phosphotransferase system has been identified in Streptococcus mutans and it was revealed that the phosphate group was on the glucose moiety.
Purification and properties of an NADP-specific 6-phosphogluconate dehydrogenase from Streptococcus faecalis.
The amino acid composition of the streptococcal enzyme is reported, and an analysis of the enzyme by sodium dodecyl sulfate polyacrylamide gel electrophoresis showed it to be a dimer composed of subunits having equal molecular weight.
Purification, resolution, and interaction of the glucosyltransferases of Streptococcus mutans 6715
The extracellular glucosyltransferase produced by Streptococcus mutans 6715 was purified from culture supernatant fluids to a specific activity of 9.6 IU/mg of protein, with an overall recovery of
Regulation and function of sucrose 6-phosphate hydrolase in Streptococcus mutans
Analysis of a mutant that was missing sucrose 6-phosphate hydrolase activity revealed that its ability to hydrolyze sucrose was reduced, and it was found that it was synthesized constitutively whereas sucrose phosphotransferase activity was inducible.
Mechanism for Regulating the Distribution of Glucose Carbon Between the Embden-Meyerhof and Hexose-Monophosphate Pathways in Streptococcus faecalis
Evidence was found that beta-mercaptoethanol blocked completely FDP inhibition, but had no effect on catalytic activity, and the possibility that the regulation of 6-phosphogluconate dehydrogenase activity by FDP might be of some general significance was suggested.
Tween 80 effect on glucosyltransferase synthesis by Streptococcus salivarius
Data strongly suggest that Tween 80 stimulates the production of extracellular GTase by acting either directly or indirectly at the level of enzyme synthesis.