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Reconciling the chemistry and biology of reactive oxygen species.
There is a vast literature on the generation and effects of reactive oxygen species in biological systems, both in relation to damage they cause and their involvement in cell regulatory and signalingExpand
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Inside the neutrophil phagosome: oxidants, myeloperoxidase, and bacterial killing.
IN THE 1880s Elie Metchnikoff observed specialized phagocytic cells ingesting bacteria, and recognized the importance of phagocytosis as a defense mechanism in multicellular organisms.[1][1]Expand
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Thiol chemistry and specificity in redox signaling.
Exposure of cells to sublethal oxidative stress results in the modulation of various signaling pathways. Oxidants can activate and inactivate transcription factors, membrane channels, and metabolicExpand
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The estimation of red cell superoxide dismutase activity.
A method is described for the estimation of red cell superoxide dismutase (erythrocuprein) and a normal range of activity established. It is likely that this enzyme is essential to the red cell forExpand
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Mitochondrial peroxiredoxin involvement in antioxidant defence and redox signalling.
Prxs (peroxiredoxins) are a family of proteins that are extremely effective at scavenging peroxides. The Prxs exhibit a number of intriguing properties that distinguish them from conventionalExpand
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Reactivity of biologically important thiol compounds with superoxide and hydrogen peroxide.
The reactivities of glutathione, cysteine, cysteamine, penicillamine, N-acetylcysteine, dithiothreitol and captopril with superoxide generated from xanthine oxidase and hypoxanthine, and with reagentExpand
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Toxicity of iron and hydrogen peroxide: the Fenton reaction.
Iron and hydrogen peroxide are capable of oxidizing a wide range of substrates and causing biological damage. The reaction, referred to as the Fenton reaction, is complex and capable of generatingExpand
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Protein carbonyl measurement by a sensitive ELISA method.
We describe a new immunoassay for measuring protein carbonyls as an index of oxidative injury. Protein samples were reacted with dinitrophenylhydrazine then adsorbed to wells of an ELISA plate beforeExpand
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Thiocyanate and chloride as competing substrates for myeloperoxidase.
The neutrophil enzyme myeloperoxidase uses H2O2 to oxidize chloride, bromide, iodide and thiocyanate to their respective hypohalous acids. Chloride is considered to be the physiological substrate.Expand
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A microtiter plate assay for superoxide dismutase using a water-soluble tetrazolium salt (WST-1).
A simple and reproducible microtiter plate assay for measuring superoxide dismutase (SOD) activity is described. Water-soluble tetrazolium, the sodium salt ofExpand
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