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Rules for optimization of biocatalysis in organic solvents
General rules for the optimization of different biocatalytic systems in various types of media containing organic solvents are derived by combining data from the literature, and the logarithm of theExpand
[16] Succinate dehydrogenase: [EC Succinate: (acceptor) oxidoreductase]
Publisher Summary The chapter describes two methods for the determination of the activity of soluble succinate dehydrogenase with artificial electrons acceptors. The first method, a manometric oneExpand
Microperoxidase/H2O2-catalyzed aromatic hydroxylation proceeds by a cytochrome-P-450-type oxygen-transfer reaction mechanism.
O2-labelled H2 18O2 demonstrated that the oxygen incorporated into aniline to give 4-aminophenol originates from H2O2, which implies that MP8, which has a histidyl and not a cysteinate fifth axial ligand, is able to react by a cytochrome-P-450-like oxygen-transfer reaction mechanism. Expand
Short-term effect of ammonium chloride on nitrogen fixation by Azotobacter vinelandii and by bacteroids of Rhizobium leguminosarum.
It is presented that the direct depressing effect of ammonium chloride on nitrogen fixation by Azotobacter vinelandii is due to inhibition of the electron transport system to nitrogenase, and its significance as a regulator for the rapid ‘switch off/switch on’ of nitrogenase activity is discussed. Expand
Substrate specificity of flavin-dependent vanillyl-alcohol oxidase from Penicillium simplicissimum. Evidence for the production of 4-hydroxycinnamyl alcohols from 4-allylphenols.
P pH-dependent binding studies suggest that vanillyl-alcohol oxidase preferentially binds the phenolate form of isoeugenol (pKa < 6, 25 degrees C). Expand
Purification and properties of glutathione reductase of human erythrocytes.
Human erythrocyte glutathione reductase (NAD(P)H) was purified 47 000-fold by column chromatography and full restoration of the activity did not guarantee that the original enzyme conformation had been acquired. Expand
The reaction mechanism of glutathione reductase from human erythrocytes.
Opposite effects of the Na+ concentration on the reaction rate of GSSG with the NADPH- and the NADH-reduced enzyme were found and NADH+ did not inhibit these reactions. Expand
Redox properties of the iron-sulfur clusters in activated Fe-hydrogenase from Desulfovibrio vulgaris (Hildenborough).
Combined elemental analysis by proton-induced X-ray emission, inductively coupled plasma mass spectrometry, instrumental neutron activation analysis, atomic absorption spectroscopy and colorimetry establishes that elements with Z > 21 (except for 12-15 Fe) are present in 0.001-0.1 mol/mol quantities, not correlating with activity. Expand
Studies on the reaction mechanism of lipoyl dehydrogenase.
Evidence is presented that the active form of the enzyme is a half-reduced form in which both flavin and the disulphide are only partially reduced, and a reaction mechanism based on these findings is proposed. Expand