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Prediction of the stability of protein mutants based on structural environment-dependent amino acid substitution and propensity tables.
An approach to the prediction of mutant stability is described using knowledge of amino acid replacements that are tolerated within the families of homologous proteins of known 3-D structure. AminoExpand
Fragment ranking in modelling of protein structure. Conformationally constrained environmental amino acid substitution tables.
Conformationally constrained environment-dependent amino acid residue substitution tables have been constructed from a database comprising 33 homologous families of protein sequences aligned on theExpand
An engineered PET depolymerase to break down and recycle plastic bottles
Present estimates suggest that of the 359 million tons of plastics produced annually worldwide 1 , 150–200 million tons accumulate in landfill or in the natural environment 2 . Poly(ethyleneExpand
Role of Glycoside Phosphorylases in Mannose Foraging by Human Gut Bacteria*
Background: The relations between the gut microbiota, food, and host play a crucial role in human health. Results: Prevalent bacterial glycoside phosphorylases are able to break down dietaryExpand
Molecular modelling of the ORL1 receptor and its complex with nociceptin.
The opioid receptor like (ORL1) receptor is a G-protein coupled receptor superfamily, and regulates a plethora of neurophysiological functions. The structural requirements for receptor activation byExpand
Functional inactivation of the nociceptin receptor by alanine substitution of glutamine 286 at the C terminus of transmembrane segment VI: evidence from a site-directed mutagenesis study of the ORL1
A site-directed mutagenesis approach has been used to gain insight into the molecular events whereby the heptadecapeptide nociceptin binds and activates the opioid receptor-like 1 (ORL1) receptor, aExpand
A generalized theoretical treatment of the kinetics of an enzyme-catalysed reaction in the presence of an unstable irreversible modifier.
  • C. Topham
  • Chemistry, Medicine
  • Journal of theoretical biology
  • 23 August 1990
A generalized theoretical treatment of the kinetics of an enzyme-catalysed reaction in the presence of an unstable irreversible inhibitor (or activator) is presented. Analytical expressionsExpand
Exploration of subsite binding specificity of human cathepsin D through kinetics and rule‐based molecular modeling
The family of aspartic proteinases includes several human enzymes that may play roles in both physiological and pathophysiological processes. The human lysosomal aspartic proteinase cathepsin D isExpand
Comparative modelling of major house dust mite allergen Der p I: structure validation using an extended environmental amino acid propensity table.
A model of the 3-D structure of a major house dust mite allergen Der p I associated with hypersensitivity reactions in humans was built from its amino acid sequence and its homology to three knownExpand
The nociceptin (ORL1) receptor: molecular cloning and functional architecture
Nociceptin and the ORL1 receptor share high sequence similarity with opioid peptides, particularly dynorphin A, and their receptors. However, nociceptin and dynorphin A may use distinct molecularExpand