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Electron spin resonance and structural analysis of water soluble, alanine-rich peptides incorporating TOAC
The unnatural, conformationally constrained nitroxide amino acid TOAC, a Cα,α-disubstituted glycine, stabilizes helical structure and provides a means for studying rigidly spin labelled peptides byExpand
A synthetic hexapeptide designed to resemble a proteinaceous p‐loop nest is shown to bind inorganic phosphate
An unexpected finding about the hexapeptide‐HPO42− complex is that the side chain ε‐amino group of the lysine occurs in its deprotonated form, which appears to bind HPO 42− via an N···H‐O‐P hydrogen bond. Expand
Molecular Recognition by a Silica-Bound Fullerene Derivative
Thermal ring opening of N-[3-(triethoxysilyl)propyl]-2-carbomethoxyaziridine in the presence of C60 produces a fulleropyrrolidine derivative which is then attached covalently to HPLC silica gel. TheExpand
The polypeptide 310-helix.
The 310-helix, first predicted as a reasonably stable polypeptide secondary structure fifty years ago, has only recently attracted the attention of structural biochemists and proteinExpand
Orientation and immersion depth of a helical lipopeptaibol in membranes using TOAC as an ESR probe.
The ESR investigation demonstrated that, in liposomes based on phosphatidylcholine, trichogin lays parallel to the membrane surface with its hydrophobic face oriented toward the membrane interior, suggesting that trichogsin might modify membrane permeability via a carpet-like mechanism, at least inliposomes and in the absence of a transmembrane potential. Expand
ESR Characterization of Hexameric, Helical Peptides Using Double TOAC Spin Labeling
α-Aminoisobutyric acid (Aib) is a Cα-tetrasubstituted amino acid that strongly favors helical structure. Most of the conformational trends established for Aib-rich peptides have been determined byExpand
Mechanism of membrane activity of the antibiotic trichogin GA IV: a two-state transition controlled by peptide concentration.
The mechanism of trichogin action can be envisaged as based on a two-state transition controlled by peptide concentration, one state is the monomeric, surface bound and inactive peptide, and the other state is a buried, aggregated form, which is responsible for membrane leakage and bioactivity. Expand
Lipid chain-length dependence for incorporation of alamethicin in membranes: electron paramagnetic resonance studies on TOAC-spin labeled analogs.
Polarity-dependent isotropic hyperfine couplings of the three TOAC derivatives indicate that alamethicin assumes approximately the same location, relative to the membrane midplane, in fluid diC(N)PtdCho bilayers with chain lengths ranging from N = 10-18. Expand
Small-amplitude backbone motions of the spin-labeled lipopeptide trichogin GA IV in a lipid membrane as revealed by electron spin echo.
This work finds that at low molar peptide to lipid ratio (1:200), where the individual peptide molecules are randomly distributed at the membrane surface, the spin labels show only a fast type of motion. Expand
(alphaMe)Nva: stereoselective syntheses and preferred conformations of selected model peptides.
This study reinforced previous conclusions that peptides based on alpha-amino acids with a Calpha-methyl substituent and aCalpha-linear alkyl substituents are characterized by a strong tendency to fold into turn and helical structures. Expand