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Sperm membrane dynamics assessed by changes in lectin fluorescence before and after capacitation.
Sperm capacitation is correlated with acquisition of fertilizing ability, and the molecular events underlying this process are only beginning to be understood. A number of membrane changes associatedExpand
Regulation of organelle transport in melanophores by calcineurin
Previous studies have shown that pigment granule dispersion and aggregation in melanophores of the African cichlid, Tilapia mossambica, are regulated by protein phosphorylation and dephosphorylation,Expand
The Initial Molecular Interaction between Mouse Sperm and the Zona Pellucida Is a Complex Binding Event*
Prior to fertilization, mammalian sperm must first bind to the zona pellucida (ZP), a glycoprotein matrix surrounding the egg. Sperm specifically bind to ZP3, an 83-kDa glycoprotein which functionsExpand
Biochemical characterization of a glycosylphosphatidylinositol-linked hyaluronidase on mouse sperm.
On the basis of DNA homology to bee venom hyaluronidase, it was recently suggested that the GPI-linked mammalian sperm antigen, PH-20, may function as a cell surface hyaluronidase [Gmachl, M., &Expand
Prostasin Is a Glycosylphosphatidylinositol-anchored Active Serine Protease*
A recombinant human prostasin serine protease was expressed in several human cell lines. Subcellular fractionation showed that this serine protease is synthesized as a membrane-bound protein while aExpand
Regulation of organelle transport: Lessons from color change in fish
Organelles transported along microtubules are normally moved to precise locations within cells. For example, synaptic vesiceles are transported to the neruronal synapse, the Golgi apparatus isExpand
Control of organelle transport in melanophores: regulation of Ca2+ and cAMP levels.
Melanophores of the cichlid Tilapia mossambica can be induced to aggregate pigment by addition of epinephrine to the medium, suggesting adrenergic control of this transport. The melanophore responseExpand
Phosphoinositide metabolism at fertilization of sea urchin eggs measured with a GFP‐probe
Fertilization elicits a dramatic, transient rise in Ca2+ within the egg which is an essential component of egg activation and consequent initiation of development. In the sea urchin egg, threeExpand
Waveform Generation Is Controlled by Phosphorylation and Swimming Direction Is Controlled by Ca2+ in Sperm from the Mosquito Culex quinquefasciatus1
ABSTRACT Most animal sperm are quiescent in the male reproductive tract and become activated after mixing with accessory secretions from the male and/or female reproductive tract. Sperm from theExpand
Microtubules and microtubule motors: mechanisms of regulation.
Microtubule-based motility is precisely regulated, and the targets of regulation may be the motor proteins, the microtubules, or both components of this intricately controlled system. Regulation ofExpand