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Emergence of new types of Theileria orientalis in Australian cattle and possible cause of theileriosis outbreaks
TLDR
Nucleotide sequencing of SSU rRNA and MPSP genes revealed the presence of four Theileria genotypes, with the occurrence of ikeda and type 4 (type C) previously not reported to occur and silent mutation is thought to have enhanced parasite evasion of the host immune response causing the outbreak.
Evolution and genetic diversity of Theileria.
TLDR
The outline of the evolution of Theileria, which includes T. orientalis, is provided, and the possible mechanisms generating genetic diversity among parasite populations are discussed, to discuss the potential implications of a genetically diverse parasite population in the context of TheILeria vaccine development.
Loop-Mediated Isothermal Amplification for Detection of African Trypanosomes
TLDR
The conditions for a highly sensitive, specific, and easy diagnostic assay based on LAMP technology for the detection of parasites in the Trypanosoma brucei group are reported, showing that the sensitivity of the LAMP-based method for detection of trypanosomes in vitro is up to 100 times higher than that of PCR-based methods.
Molecular epidemiology of camel trypanosomiasis based on ITS1 rDNA and RoTat 1.2 VSG gene in the Sudan
TLDR
It is concluded that camel trypanosomiasis in Sudan is apparently caused by a single parasite species T. evansi and there were no other typanosomes species detected.
A study of the systematics of Theileria spp. based upon small-subunit ribosomal RNA gene sequences
TLDR
The current results show that the United States bovine Theileria isolates are not T. mutans because they have T. buffeli Warwick and T.buffeli Marula (Type A) and/or Type D (species undesignated) SSU rRNA gene sequences.
Infection of bovine immunodeficiency virus and bovine leukemia virus in water buffalo and cattle populations in Pakistan.
TLDR
BIV-seropositive waterbuffaloes and cattle were found to have BIV proviral DNA in the peripheral blood mononuclear cells determined by nested polymerase chain reaction, the first report of BIV infections in water buffaloes.
Serodiagnosis of Toxoplasma gondii infection in cats by enzyme-linked immunosorbent assay using recombinant SAG1.
TLDR
The gene encoding surface antigen 1 (SAG1, P30) of Toxoplasma gondii was cloned into the plasmid pGEX-4T-3 and subsequently expressed in Escherichia coli as a glutathione-S-transferase (GST) fusion protein, which was evaluated in an enzyme-linked immunosorbent assay (ELISA) for serological diagnosis of toxoplasmosis.
Apical membrane antigen 1 is a cross-reactive antigen between Neospora caninum and Toxoplasma gondii, and the anti-NcAMA1 antibody inhibits host cell invasion by both parasites.
TLDR
Results indicated that AMA1 is a cross-reactive antigen between N. caninum and T. gondii and a potential common vaccine candidate to control two parasites.
Microbial Population Analysis of the Salivary Glands of Ticks; A Possible Strategy for the Surveillance of Bacterial Pathogens
TLDR
The microbial populations in tick salivary glands were different between tick species, indicating that host specificities may play an important role in determining the microbial complement.
Genotypic diversity of Theileria orientalis detected from cattle grazing in Kumamoto and Okinawa prefectures of Japan.
TLDR
The results indicate that at least 5 types of T. orientalis exist in Kumamoto and Okinawa prefectures of Japan and that genotype-specific PCR assays are highly applicable for the quarantine of transported cattle and for epidemiological surveys of bovine theileriosis in Japan.
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