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Quantitative Ultrastructural Analysis of Hippocampal Excitatory Synapses
The distribution of active zone areas can account for the distribution of synaptic release probabilities and that each active zone constitutes a release site as identified in the standard quantal theory attributable to Katz (1969). Expand
Heterogeneity of Release Probability, Facilitation, and Depletion at Central Synapses
This study uses recordings from putative single synaptic release sites in hippocampal slices to show that significant heterogeneity exists in facilitation and depletion among synapses, and demonstrates that the release probability at a synapse is directly correlated with the size of its readily releasable vesicle pool. Expand
Voltage dependence of NMDA-activated macroscopic conductances predicted by single-channel kinetics
  • C. Jahr, C. Stevens
  • Chemistry, Medicine
  • The Journal of neuroscience : the official…
  • 1 September 1990
The conductance activated in many mammalian CNS neurons by the glutamate analog NMDA is inhibited at hyperpolarized potentials by extracellular magnesium, consistent with the assumption that magnesium inhibits current through the NMDA-activated channel by directly blocking the ion pore. Expand
Definition of the Readily Releasable Pool of Vesicles at Hippocampal Synapses
It is found that hypertonic solutions do not act through changes in intracellular calcium, which means that the synaptic release probability depends on the size of the readily releasable pool. Expand
Synaptotagmin I: A major Ca2+ sensor for transmitter release at a central synapse
It is proposed that synaptotagmin I is the major low affinity Ca2+ sensor mediating Ca2-regulation of synchronous neurotransmitter release in hippocampal neurons and not essential for asynchronous or Ca(2+)-independent release. Expand
Inactivity Produces Increases in Neurotransmitter Release and Synapse Size
The physiologically measured size of the readily releasable pool (RRP) and the release probability are increased and the same number of vesicles are docked as are estimated to be in the RRP within measurement error. Expand
Heterogeneous Release Properties of Visualized Individual Hippocampal Synapses
Endocytotic uptake of the styryl dye FM1-43 at synaptic terminals is used to study properties of individual synapses formed by axons of single hippocampal neurons in tissue culture, demonstrating that the optically estimated distribution of p at autapses in single-neuron microislands predicts the rate of blocking of NMDA responses by the noncompetitive antagonist MK-801 at the same synapses. Expand
Two components of transmitter release at a central synapse.
  • Y. Goda, C. Stevens
  • Medicine, Biology
  • Proceedings of the National Academy of Sciences…
  • 20 December 1994
The findings are comparable to those of the classical studies for the frog neuromuscular junction, and thus the basic aspects of Ca(2+)-activated transmitter release machinery appear to be conserved in central synapses. Expand
Three modes of synaptic vesicular recycling revealed by single-vesicle imaging
It is shown that an exocytic event can terminate with three modes of vesicle retrieval: a fast ‘kiss-and-run’ mode that has a selective fusion pore; a slow (8–21 s) ‘compensatory” mode; and a ‘stranded’ modes of recycling, in which a vesicles is left on the cell surface until a nerve impulse triggers its retrieval. Expand
Deficient hippocampal long-term potentiation in alpha-calcium-calmodulin kinase II mutant mice.
W Whole cell recordings reveal that postsynaptic mechanisms, including N-methyl-D-aspartate (NMDA) receptor function, are intact and are therefore a suitable model for studying the relation between LTP and learning processes. Expand