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Molecular mechanisms of biocatalytic desulfurization of fossil fuels
TLDR
A unique sulfur acquisition system that Rhodococcus uses to obtain sulfur from very stable heterocyclic molecules is described, with no apparent sequence homology to any other protein in the databases.
Genetic analysis of the dsz promoter and associated regulatory regions of Rhodococcus erythropolis IGTS8
TLDR
The dsz gene cluster of Rhodococcus erythropolis IGTS8 comprises three genes, ds z, whose products are involved in the conversion of dibenzothiophene to 2-hydroxybiphenyl and sulfite, which can use DBT as the sole sulfur source but not as a carbon source.
DNA shuffling method for generating highly recombined genes and evolved enzymes
TLDR
This method represents a conceptually distinct and improved alternative to sexual PCR for gene family shuffling and is demonstrated by molecular breeding of a monooxygenase for increased rate and extent of biodesulfurization on complex substrates, as well as for 20-fold faster conversion of a nonnatural substrate.
Biodesulfurization of fossil fuels.
A flavin reductase stimulates DszA and DszC proteins of Rhodococcus erythropolis IGTS8 in vitro.
TLDR
It is demonstrated that a FMN:NADPH oxidoreductase from Vibrio harveyi complements activities of purified DszA and DsZC proteins, and it is proposed that Ds zA andDszC are oxygenase units that do not use NAD(P)H directly, but instead use FMNH2 from a FM nadPH oxidorductase for oxygenation.
A second tumor necrosis factor receptor gene product can shed a naturally occurring tumor necrosis factor inhibitor.
TLDR
An inhibitor of tumor necrosis factor (TNF) has been isolated from the human histiocytic lymphoma cell line U-937 that is capable of inhibiting both TNF-alpha and T NF-beta and is distinct from a previously described TNF inhibitor that is a soluble fragment of a TNF receptor molecule (TNFrI).
Shuttle plasmids for Escherichia coli and Clostridium perfringens
TLDR
Reciprocal transformation experiments in which plasmid DNAs derived from E. coli or C. perfringens were used suggest that restriction barriers exist between these two organisms, and contain restriction enzyme recognition sites in locations which are useful for cloning experiments.
Multifunctional regulation of the biological effects of TNF-alpha by the soluble type I and type II TNF receptors.
TLDR
The rsTNF-RI was chosen as the better candidate for development as an anti-inflammatory agent after a murine model of cytokine-induced shock provided protection against the lethality of shock induced by a synergistic combination of rhT NF-alpha and rhIL-1 beta.
IlvHI locus of Salmonella typhimurium
TLDR
Results, together with results from other laboratories, suggest that S. typhimurium has functional ilvB and ilvG genes and a cryptic ilvHI locus and E. coli K-12, on the other hand, hasfunctional ilv B and ilVHI genes and an cryptic ilVG locus.
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