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When should a DDH experiment be mandatory in microbial taxonomy?

Whether or not, and in which situations, this threshold value might be too conservative, is investigated, which means up to half of the currently conducted DDH experiments could safely be omitted without a significant risk for wrongly differentiated species.
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Chryseobacterium hispalense sp. nov., a plant-growth-promoting bacterium isolated from a rainwater pond in an olive plant nursery, and emended descriptions of Chryseobacterium defluvii,…

A novel non-motile, Gram-staining-negative, yellow-pigmented bacterium, designated AG13(T), isolated from a rain water pond at a plant nursery in Spain and characterized as a plant-growth-promoting…
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Characterization of the first cultured representative of Verrucomicrobia subdivision 5 indicates the proposal of a novel phylum

It is proposed that strain L21-Fru-ABT and related bacteria are specifically adapted to the utilization of sulfated glycopolymers produced in microbial mats or biofilms.
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Global outbreak of severe Mycobacterium chimaera disease after cardiac surgery: a molecular epidemiological study.

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Pedobacter cryoconitis sp. nov., a facultative psychrophile from alpine glacier cryoconite.

A facultatively psychrophilic, Gram-negative, aerobic, rod-shaped strain, A37(T), was isolated from alpine glacier cryoconite and showed activities of oxidase, catalase, DNase, protease (gelatin, casein), amylase, beta-glucosidase and beta-lactamase and degraded oil hydrocarbons.
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Characterisation, genome size and genetic manipulation of the myxobacterium Sorangium cellulosum So ce56

S. cellulosum So ce56 was phenotypically and genotypically analysed in order to evaluate whether this strain can be used in a comprehensive genome project as a representative of the secondary metabolite-producing myxobacteria, and was found to have various advantageous features.
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Characterization of Vibrio viscosus and Vibrio wodanis isolated at different geographical locations: a proposal for reclassification of Vibrio viscosus as Moritella viscosa comb. nov.

The results revealed that the V. viscosus and V. wodanis strains are heterogeneous genotypically and phenotypically, and a reclassification of Vibrio Viscosus as Moritella viscosa comb is proposed.
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The phylogenetic position of Serratia, Buttiauxella and some other genera of the family Enterobacteriaceae.

The phylogenetic relationships of the type strains of 38 species from 15 genera of the family Enterobacteriaceae were investigated by comparative 16S rDNA analysis, finding that Buttiauxella and Citrobacter share type strains greater than 99%.
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Molecular biological evidence for the occurrence of uncultured members of the actinomycete line of descent in different environments and geographical locations.

Comparison with short 16S rDNA clone sequences obtained from DNA isolated from soil in Australia, Japan and Finland and marine environments from the Atlantic and the Pacific Oceans, suggests that members of these three groups occur in very different environments across the world.
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Halobacillus karajensis sp. nov., a novel moderate halophile.

It is proposed that strain MA-2T should be placed in the genus Halobacillus as the type strain of a novel species, HalOBacillus karajensis sp.
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