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A therapy-grade protocol for differentiation of pluripotent stem cells into mesenchymal stem cells using platelet lysate as supplement
TLDR
This work presents a therapy-grade, inexpensive and simple protocol to derive MSCs from pluripotent stem cells (PSCs) based on the use of platelet lysate (PL) as medium supplement and shows that it can be used to generate a large amount of PD-MSCs with low cost and is compatible with clinical therapies. Expand
EDA-Containing Fibronectin Increases Proliferation of Embryonic Stem Cells
TLDR
It is shown for the first time that this FN isoform enhances ESC’s proliferation, suggesting a possible conserved behavior for regulation of ES cells proliferation by this FNisoform and could contribute to improve their culturing conditions both for research and cell therapy. Expand
Modulation of chromatin modifying factors' gene expression in embryonic and induced pluripotent stem cells.
TLDR
A deeper knowledge of the epigenetic regulation of ESC will allow improving somatic cell reprogramming for iPSC obtention and differentiation protocols optimization, and could contribute to the comprehension of molecular mechanisms involved in pluripotency, early differentiation and reprograming. Expand
Identification of virulence markers in clinically relevant strains of Acinetobacter genospecies.
TLDR
Nine Acinetobacter strains from patients and hospital environment were analyzed for virulence markers, quorum sensing signal production, and the presence of luxI and luxR genes, and all members of the so-called A. baumannii complex, regardless of whether the source was a patient or environmental, secreted mediumto long-chain N-acyl homoserine lactones and showed blue light inhibition of cell motility. Expand
Protein arginine Methyltransferase 8 gene is expressed in pluripotent stem cells and its expression is modulated by the transcription factor Sox2.
TLDR
Findings suggest that besides its known function in nervous system, Prmt8 could play a role in pluripotent stem cells, and its transcription is modulated by pluripotency transcription factors. Expand
Inhibition of Cell Division and DNA Replication Impair Mouse-Naïve Pluripotency Exit.
TLDR
It is demonstrated that impairment of DNA replication severely blocks transcriptional switch to primed pluripotency, even in the absence of p53 activity induced by the DNA damage response, which suggests an important role for DNA replication during mouse embryonic stem cell differentiation. Expand
Extracellular vesicles from pluripotent stem cell-derived mesenchymal stem cells acquire a stromal modulatory proteomic pattern during differentiation
TLDR
Findings suggest that during differentiation, compared with their parental iPSC EVs, PD-MSC EVs acquire a more specific set of proteins; arguably, this difference might confer their therapeutic properties. Expand
Maintenance of murine embryonic stem cells' self-renewal and pluripotency with increase in proliferation rate by a bovine granulosa cell line-conditioned medium.
TLDR
This work found that the conditioned medium from a bovine granulosa cell line (BGC-CM) is able to maintain mESCs' self-renewal while preserving its critical properties without LIF addition, and this medium has an increased proliferation rate compared with cells grown in the mESC standard culture medium supplemented with LIF. Expand
Manganese Superoxide Dismutase Gene Expression Is Induced by Nanog and Oct4, Essential Pluripotent Stem Cells’ Transcription Factors
TLDR
The results indicate that pluripotency transcription factors positively modulate Sod2 gene transcription, and this work has found that Manganese superoxide dismutase gene was repressed during diverse differentiation protocols showing an expression pattern similar to Nanog gene. Expand
Induced pluripotent stem cells' self-renewal and pluripotency is maintained by a bovine granulosa cell line-conditioned medium.
TLDR
Reprogrammed mouse embryonic fibroblasts obtaining iPSC lines are reprogrammed and it is shown that they can be propagated in BGC-CM while maintaining self-renewal and pluripotency, evidenced by expression of specific gene markers and capability of in vitro and in vivo differentiation to cell types from the three germ layers. Expand
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