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Molecular and Biochemical Characterization of a Highly Stable Bacterial Laccase That Occurs as a Structural Component of theBacillus subtilis Endospore Coat*
TLDR
This model of CotA contains all the structural features of a laccase, including the reactive surface-exposed copper center (T1) and two buried copper centers (T2 and T3), and shows a half-life of inactivation at 80 °C of about 4 and 2 h, indicating that CotA is intrinsically highly thermostable.
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Evolution of the thyroid hormone-binding protein, transthyretin.
TLDR
Protein modelling studies are presented that demonstrate differences in the electrostatic characteristics of the molecule in human, rat, chicken, and fish, which may explain why, in contrast to TTR from human and rat, T TR from fish and birds preferentially binds triiodo-l-thyronine.
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Mind the gap: cytochrome interactions reveal electron pathways across the periplasm of Shewanella oneidensis MR-1.
TLDR
The results showed that both STC and FccA interact with their redox partners, CymA and MtrA, through a single haem, avoiding the establishment of stable redox complexes capable of spanning the periplasmic space.
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Functional Domains of the Bacillus subtilis Transcription Factor AraR and Identification of Amino Acids Important for Nucleoprotein Complex Assembly and Effector Binding
TLDR
This work uses random mutagenesis, deletion, and construction of chimeric LexA-AraR fusion proteins to map the functional domains of AraR required for DNA binding, dimerization, and effector binding and provides insight into the mechanistic mode of action of this modular repressor.
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Constant-pH molecular dynamics using stochastic titration
A new method is proposed for performing constant-pH molecular dynamics (MD) simulations, that is, MD simulations where pH is one of the external thermodynamic parameters, like the temperature or the…
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Crystal Structure of Cardosin A, a Glycosylated and Arg-Gly-Asp-containing Aspartic Proteinase from the Flowers ofCynara cardunculus L.*
TLDR
Based on the crystal structure, a possible mechanism whereby cardosin A might be orientated at the cell surface of the style to interact with its putative receptor from pollen is proposed.
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Sulphate respiration from hydrogen in Desulfovibrio bacteria: a structural biology overview.
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Proximal mutations at the type 1 copper site of CotA laccase: spectroscopic, redox, kinetic and structural characterization of I494A and L386A mutants.
TLDR
Overall the data clearly indicate that the Ile(494) mutation causes a major alteration of the structure near the type 1 copper site and this has been confirmed by X-ray crystallography.
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Structural consequences of ATP hydrolysis on the ABC transporter NBD dimer: Molecular dynamics studies of HlyB
TLDR
A molecular dynamics study on the NBD dimer of the HlyB ABC exporter reveals a change in the contact surface between the monomers after hydrolysis, and proposes that the X‐loop motif of ABC exporters, which has been shown to be in contact with the coupling helices of the transmembrane domains (TMDs), is an important part of the Nbd‐TMD communication in ABC exporers.
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Protein structure and dynamics in ionic liquids. Insights from molecular dynamics simulation studies.
TLDR
This work presents the first molecular simulation study of an enzyme, the serine protease cutinase from Fusarium solani pisi, in two ionic liquids (ILs), and shows that these ILs "strip off" most of the water from the enzyme surface in a degree similar to that found for polar organic solvents such as acetonitrile.
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