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Two-Helper RNA System for Production of Recombinant Semliki Forest Virus Particles
A new packaging system for Semliki Forest virus (SFV) based on a the use of a two-helper system in which the capsid and spike proteins of the C-p62-6K-E1 polyprotein are expressed from two independent RNA molecules. Expand
Replication and Packaging of Transmissible Gastroenteritis Coronavirus-Derived Synthetic Minigenomes
The synthetic minigenomes used to design a helper-dependent expression system that produces around 1.0 μg/106 cells of GUS, and the use of the reporter gene β-glucuronidase allowed minigenome detection at passage zero, making it possible to distinguish replication efficiency from packaging capability. Expand
Enhancing immune responses using suicidal DNA vaccines
We describe a DNA vaccine strategy that allows antigens to be produced in vivo in the context of an alphaviral replicon. Mice immunized with such vectors developed humoral and cellular immuneExpand
Antigenic homology among coronaviruses related to transmissible gastroenteritis virus
The classification of the human coronavirus 229E in a taxonomic cluster distinct from TGEV group is suggested because an antigenic subsite involved in neutralization was conserved in porcine, feline, and canine coronav virus. Expand
Long noncoding RNA EGOT negatively affects the antiviral response and favors HCV replication
The results suggest that EGOT is a lncRNA induced after infection that increases viral replication by antagonizing the antiviral response, providing a novel link between HCV infection and development of liver tumors. Expand
Immunization with recombinant Semliki Forest virus induces protection against influenza challenge in mice.
Immunization of mice with vectors encoding the Influenza A virus antigens nucleoprotein (NP) and hemagglutinin (HA) resulted in immune responses that were protective against challenge infection with Influenza virus. Expand
Mechanisms of transmissible gastroenteritis coronavirus neutralization
Transmissible gastroenteritis virus (TGEV) was neutralized more than 109-fold with antibodies of a single specificity [monoclonal antibodies (MAbs]; Mechanisms of TGEV neutralization by MAbs were characterized at high, intermediate, and low antibody-to-virus ratios. Expand
Semliki Forest Virus Expressing Interleukin-12 Induces Antiviral and Antitumoral Responses in Woodchucks with Chronic Viral Hepatitis and Hepatocellular Carcinoma
Experimental observations suggest that intratumoral administration of SFV-enhIL-12 may represent a strategy for treatment of chronic HBV infection and associated HCC in humans but indicate that this approach could benefit from further improvements. Expand
PDL1 Signals through Conserved Sequence Motifs to Overcome Interferon-Mediated Cytotoxicity.
PDL1 cell-intrinsic signaling protects cancer cells from interferon (IFN) cytotoxicity and accelerates tumor progression and reveals a mode of action of PDL1 in cancer cells as a first line of defense against IFN cytot toxicity. Expand
Molecular Characterization of Transmissible Gastroenteritis Coronavirus Defective Interfering Genomes: Packaging and Heterogeneity
Heterogeneity maps at the beginning of the S gene, where other natural TGEV recombination events have been observed, suggesting that either a process of template switching occurs with high frequency at this point or that the derived genomes have a selective advantage. Expand