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A single Photorhabdus gene, makes caterpillars floppy (mcf), allows Escherichia coli to persist within and kill insects
It is shown that a single large Photorhabdus gene, makes caterpillars floppy (mcf), is sufficient to allow Esherichia coli both to persist within and kill an insect. Expand
Resolution and partial characterization of proteinases and α-amylases from midguts of larvae of the bruchid beetle Callosobruchus maculatus (F.)
Three proteinases and four α-amylases wre identified in Callosobruchus maculatus larvae have the same M r (25,000) and optimum pH between 5.5 and 6.0 and are inhibited by iodoacetic acid, iodoacetamide, pCMB, TLCK, TPCK and E-64. Expand
Poor correlation between the levels of proteinase inhibitors found in seeds of different cultivars of cowpea (Vigna unguiculata) and the resistance/susceptibility to predation by Callosobruchus
The C. maculatus resistant cowpea seeds support the development of Zabrotes subfasciatus, a bruchid that infests seeds of both Phaseolus vulgaris and Vigna unguiculata. Expand
Activity of wheat alpha-amylase inhibitors towards bruchid alpha-amylases and structural explanation of observed specificities.
Improved structural understanding of the bases for the 0.19 structural family inhibitor specificity reported here may prove useful in the future for the rational design of inhibitors possessing altered inhibition characteristics. Expand
Proteinases and amylases of larval midgut of Zabrotes subfasciatus reared on cowpea (Vigna unguiculata) seeds
Proteinase and amylase activities in larval midguts of the bruchid beetle Zabrotes subfasciatus (Boh.) (Coleoptera: Bruchidae) reared on cowpea seeds were investigated and it was found that the aspartic class proteolytic activity is higher than the cysteine class proteinase activity in the midgut of Z. subfAsciatus. Expand
Comparison between the levels of aspartic and cysteine proteinases of the larval midguts of Callosobruchus maculatus (F.) and Zabrotes subfasciatus (BOH.) (Coleoptera: bruchidae)
An acidic proteolytic enzyme acting on hemoglobin at optimum pH 3.3 was partially purified from Callosobruchus maculatus larval midgut and suggests that this bruchid has a higher capacity to digest proteins of some cowpea seeds than C.maculatus has. Expand
Digestion of legume starch granules by larvae of Zabrotes subfasciatus (Coleoptera: bruchidae) and the induction of alpha-amylases in response to different diets.
A detailed study on the digestive process of the granules was conducted, and in vitro hydrolysis rates of purified granules and the pattern of dextrins liberated by the different alpha-amylases were similar for the two legume species. Expand
Digestion in larvae of Callosobruchus maculatus and Zabrotes subfasciatus (Coleoptera: Bruchidae) with emphasis on α-amylases and oligosaccharidases
The data suggest that the majority of carbohydrate digestion occurs in the midgut lumen, whereas protein digestion should take place partly in the lumen and partly at the cell surface, which is qualitatively appropriate to digest the free oligosaccharides and oligomaltodextrins produced by α-amylases from the starch granules of host seeds. Expand
Cowpea (Vigna unguiculata) vicilins bind to the peritrophic membrane of larval sugarcane stalk borer (Diatraea saccharalis).
In this work, we show that vicilins from two Vigna unguiculata (cowpea) genotypes, Epace-10 and IT 81D-1045, which are susceptible and resistant to attack by the cowpea weevil CallosobruchusExpand
Records of Guiana dolphin, Sotalia guianensis , in the State of Ceará, Northeastern Brazil
The Guiana dolphin, Sotalia guianensis , is a small delphinid found in costal waters, estuaries and bays from Santa Catarina, Brazil, to Nicaragua. Strandings of Guiana dolphins were recordedExpand