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A Mec1- and Rad53-dependent checkpoint controls late-firing origins of DNA replication
DNA replication in eukaryotic cells initiates from many replication origins which fire throughout the S phase of the cell cycle in a predictable pattern: some origins fire early, others late. LittleExpand
An essential role for the Cdc6 protein in forming the pre-replicative complexes of budding yeast
ORIGINS of DNA replication in Saccharomyces cerevisiae are bound by two protein complexes during the cell cycle1,2. Post-replicative complexes closely resemble those generated in vitro by purifiedExpand
Identification of Mcm2 Phosphorylation Sites by S-phase-regulating Kinases*
Minichromosome maintenance 2-7 proteins play a pivotal role in replication of the genome in eukaryotic organisms. Upon entry into S-phase several subunits of the MCM hexameric complex areExpand
A Cdc7 kinase inhibitor restricts initiation of DNA replication and has antitumor activity.
Cdc7 is an essential kinase that promotes DNA replication by activating origins of replication. Here, we characterized the potent Cdc7 inhibitor PHA-767491 (1) in biochemical and cell-based assays,Expand
Cdc7 Inhibition Reveals a p53-Dependent Replication Checkpoint That Is Defective in Cancer Cells
Cdc7 is an evolutionarily conserved kinase that regulates S phase by promoting replication origin activation. Down-regulation of Cdc7 by small interfering RNA in a variety of tumor cell lines causesExpand
ORC‐ and Cdc6‐dependent complexes at active and inactive chromosomal replication origins in Saccharomyces cerevisiae.
We have developed a genomic footprinting protocol which allows us to examine protein‐DNA interactions at single copy chromosomal origins of DNA replication in the budding yeast SaccharomycesExpand
Cdc7 Is an Active Kinase in Human Cancer Cells Undergoing Replication Stress*
Cdc7 kinase promotes and regulates DNA replication in eukaryotic organisms. Multiple mechanisms modulating kinase activity in response to DNA replication stress have been reported, supporting theExpand
Activation of dormant origins of DNA replication in budding yeast.
Eukaryotic genomes often contain more potential replication origins than are actually used during S phase. The molecular mechanisms that prevent some origins from firing are unknown. Here we showExpand
Dbf4p, an Essential S Phase-Promoting Factor, Is Targeted for Degradation by the Anaphase-Promoting Complex
ABSTRACT The Dbf4p/Cdc7p protein kinase is essential for the activation of replication origins during S phase. The catalytic subunit, Cdc7p, is present at constant levels throughout the cell cycle.Expand
DNA mediated chromatin pull-down for the study of chromatin replication
Chromatin replication involves duplicating DNA while maintaining epigenetic information. These processes are critical for genome stability and for preserving cell-type identity. Here we describe aExpand