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The Amyloid Precursor Protein Has a Flexible Transmembrane Domain and Binds Cholesterol
NMR titration of C99 reveals a binding site for cholesterol, providing mechanistic insight into how cholesterol promotes amyloidogenesis and may aid in the design of Alzheimer’s therapeutics.
Characterization of magnetically orientable bilayers in mixtures of dihexanoylphosphatidylcholine and dimyristoylphosphatidylcholine by solid-state NMR.
The information gleaned about these mixtures and the quality of the oriented NMR spectra obtained suggest that DHPC-DMPC mixtures may prove to be useful as model membrane media in solid-state NMR studies of biomembranes.
Magnetically-oriented phospholipid micelles as a tool for the study of membrane-associated molecules
Structure of KCNE1 and implications for how it modulates the KCNQ1 potassium channel.
Docking to open KCNQ1 indicates that the extracellular end of the KCNE1 TMD forms an interface with an intersubunit cleft in the channel that is associated with most known gain-of-function disease mutations.
Solution Nuclear Magnetic Resonance Structure of Membrane-Integral Diacylglycerol Kinase
The three-dimensional structure of the DAGK homotrimer is determined with the use of solution nuclear magnetic resonance, providing insight into the determinants of lipid substrate specificity and phosphotransferase activity.
Bicelles: a model membrane system for all seasons?
Substrate specificity of γ-secretase and other intramembrane proteases
A large body of emerging data allows us to survey the substrates of γ-secretase to ascertain the conformational features that predispose a peptide to cleavage by this enigmatic protease, and surveys the voluminous body of literature concerning the traffic of ιsecretase and its most prominent substrate, the amyloid precursor protein.
Direct binding of cholesterol to the amyloid precursor protein: An important interaction in lipid-Alzheimer's disease relationships?
Reconstitution of membrane proteins into lipid-rich bilayered mixed micelles for NMR studies.
Results indicate that bicelles may be uniquely and effectively employed as model membranes to facilitate NMR structural studies of many, but not all, membrane proteins.
Solution NMR of membrane proteins: practice and challenges
- C. Sanders, F. Sönnichsen
- Chemistry, Environmental ScienceMagnetic resonance in chemistry : MRC
- 1 July 2006
This review focuses upon the application of solution NMR methods to multispan integral membrane proteins, particularly with respect to determination of global folds by this approach. Practical…