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Probing the cathepsin D using a BODIPY FL-pepstatin A: applications in fluorescence polarization and microscopy.
- C. S. Chen, W. Chen, M. Zhou, S. Arttamangkul, R. Haugland
- Chemistry, Medicine
- Journal of biochemical and biophysical methods
- 16 March 2000
Redistribution of cathepsin D, a major lysosomal aspartic endopeptidase, has been related to various pathological progressions during tumor formation and oxidation stress. We have synthesized a… Expand
Redox-dependent trafficking of 2,3,4,5, 6-pentafluorodihydrotetramethylrosamine, a novel fluorogenic indicator of cellular oxidative activity.
The trafficking of 2,3,4,5,6-pentafluorodihydrotetramethylrosamine (PF-H(2)TMRos, also known as RedoxSensor Red), a new fluorogenic indicator for oxidative activity, was evaluated in a… Expand
Purification and characterization of a proteinase identified as cathepsin D from tilapia muscle (Tilapia nilotica .times. Tilapia aurea)
For investigating which pepstatin-sensitive proteinases are involved in post-mortem myofibril degradation, the cathepsin D was first purified and characterised
Role of pepstatin-sensitive proteases on the postmortem changes of tilapia (Tilapia nilotica .times. Tilapia aurea) muscle myofibrils
To investigate the pepstatin-sensitive proteases in fish muscle tenderization, tilapia myofibrils (G-I), myofibrils with crude proteases added (G-II), and myofibrils with crude proteases and… Expand
Denaturation and change in sulfhydryl group of actomyosin from milkfish (Chanos chanos) during storage at -20.degree.C
Au cours de la congelation et de l'entreposage a l'etat congele, le mecanisme de denaturation est principalement cause par la formation de liaisons hydrogene, hydrophobes et disulfure
Effect of storage temperatures on the formation of disulfides and denaturation of milkfish myosin (Chanos chanos)
Etude de l'effet des temperatures de congelation (-20 et -35 degres C pendant 8 semaines) sur la denaturation des proteines de ce poisson. Etude de l'activite enzymatique de la Mg- et Mg(Ca)-ATPases,… Expand
Purification and characterization of proteases from milkfish muscle (Chanos chanos)
To investigate the effects of storage methods on proteolysis of fish muscle and viscera, and subsequently the mechanism of maintaining quality by these storage methods, proteases from milkfish muscle… Expand