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Phosphorylated Pol II CTD recruits multiple HDACs, including Rpd3C(S), for methylation-dependent deacetylation of ORF nucleosomes.
Methylation of histone H3 by Set1 and Set2 is required for deacetylation of nucleosomes in coding regions by histone deacetylase complexes (HDACs) Set3C and Rpd3C(S), respectively. We report thatExpand
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Nucleosome contact triggers conformational changes of Rpd3S driving high-affinity H3K36me nucleosome engagement.
The Rpd3S histone deacetylase complex utilizes two subunits, Eaf3 and Rco1, to recognize nucleosomes methylated at H3K36 (H3K36me) with high affinity and strong specificity. However, the chromobarrelExpand
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Fluorescence energy transfer dye-labeled primers for DNA sequencing and analysis.
Fluorescent dye-labeled DNA primers have been developed that exploit fluorescence energy transfer (ET) to optimize the absorption and emission properties of the label. These primers carry aExpand
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Combinatorial Histone Readout by the Dual Plant Homeodomain (PHD) Fingers of Rco1 Mediates Rpd3S Chromatin Recruitment and the Maintenance of Transcriptional Fidelity*
The plant homeodomain (PHD) finger is found in many chromatin-associated proteins and functions to recruit effector proteins to chromatin through its ability to bind both methylated and unmethylatedExpand
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The DNA Repair Protein yKu80 Regulates the Function of Recombination Enhancer during Yeast Mating Type Switching
ABSTRACT Recombination enhancer (RE) is essential for regulating donor preference during yeast mating type switching. In this study, by using minichromosome affinity purification (MAP) and massExpand
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Thermo Sequenase DNA polymerase and T. acidophilum pyrophosphatase: new thermostable enzymes for DNA sequencing.
A combination of thermostable enzymes has been developed that produces higher quality cycle sequences. Thermo Sequenase DNA polymerase is a thermostable enzyme engineered to catalyze theExpand
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Reconstitution of modified chromatin templates for in vitro functional assays.
To study the functions of histone modifications in the context of chromatin, it is necessary to be able to prepare nucleosomal templates that carry specific posttranslational modifications in aExpand
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Purification of native, defined chromatin segments.
Publisher Summary This chapter provides an approach to learn the composition, structure, and function of unique genes packaged as chromatin in different functional states. The approach has beenExpand
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Design and synthesis of fluorescence energy transfer dye-labeled primers and their application for DNA sequencing and analysis.
We have designed and synthesized fluorescent oligonucleotide primers having improved fluorescence and electrophoretic properties by exploiting the concept of resonance fluorescence energy transferExpand
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