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The structure of a complex of recombinant hirudin and human alpha-thrombin.
The crystallographic structure of a recombinant hirudin-thrombin complex has been solved at 2.3 angstrom (A) resolution and abundant interactions may account for the high affinity and specificity of hirUDin. Expand
The glutathione transferase activity and tissue distribution of a cloned Mr28K protective antigen of Schistosoma mansoni.
A protective Mr28K antigen of Schistosoma mansoni, expressed from its cDNA, has been purified in a single step and shown to possess glutathione (GSH) transferase activity as predicted from sequenceExpand
Analysis of the lipidated recombinant outer surface protein A from Borrelia burgdorferi by mass spectrometry.
The mass analysis of the major mass is consistent with a mixed population of lipoprotein molecules containing variations not only in the lipid moiety contributing to an elevation in the mass of Deltam = 7 Da compared to the theoretical structure proposed, but also in the peptide chain. Expand
Bacillopeptidase F: two forms of a glycoprotein serine protease from Bacillus subtilis 168
The data suggest that bacillopeptidase F is distinct from all other proteases of B. subtilis. Expand
Altered specificities of genetically engineered alpha 1 antitrypsin variants.
Seven active site variants of human alpha 1-antitrypsin (alpha 1AT) produced in Escherichia coli following site-specific mutagenesis of the alpha 1AT complementary DNA have therapeutic potential and could be effective in the control of thrombosis. Expand
Expression and Secretion in S. Cerevisiae of Biologically Active Leech Hirudin
Only precursors in which the HV2 mature sequence directly followed the cleavage site for yscF (a protease involved in the maturation of yeast α factor) were processed correctly to yield biologically active hirudin. Expand
High level of expression of a protective antigen of schistosomes in Saccharomyces cerevisiae
Strains of Saccharomyces cerevisiae expressing P28‐I, an antigen inducing protection against schistosomiasis, have been constructed using the ura3 fur1 auto‐selection system and constitutive and stable expression of P 28‐I could be obtained in cultures grown in rich medium. Expand
Purification and biochemical characterization of recombinant alpha 1-antitrypsin variants expressed in Escherichia coli.
The isolated variants of alpha 1AT did not show any signs of proteolytic degradation during the purification process and proved to be fully active against their target proteases, opening the possibility to evaluate these novel protease inhibitors for their in vivo efficacy in different animal models of human disease. Expand
Inhibition by recombinant hirudins of experimental venous thrombosis and disseminated intravascular coagulation induced by tissue factor in rats.
Recombinant hirudin rHV2-Lys47 appears as a promising potent antithrombotic agent for the prevention of thrombin-dependent venous thrombosis and disseminated intravascular coagulation. Expand
Characterization of the deamidated forms of recombinant hirudin.
Recombinant hirudin variant rHV2-Lys 47 was intentionally deamidated by incubation in pH 9 phosphate buffer at 37 degrees C and acid-catalyzed carboxyl methylation enhanced, in particular, the abundance of the sequence ions in the LSIMS spectra. Expand