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A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.
- S. Tabor, C. Richardson
- Biology, Medicine
- Proceedings of the National Academy of Sciences…
- 1 February 1985
The RNA polymerase gene of bacteriophage T7 has been cloned into the plasmid pBR322 under the inducible control of the lambda PL promoter. After induction, T7 RNA polymerase constitutes 20% of the… Expand
Performance of electron reconstruction and selection with the CMS detector in proton-proton collisions at √s=8 TeV
The performance and strategies used in electron reconstruction and selection at CMS are presented based on data corresponding to an integrated luminosity of 19.7 inverse femtobarns, collected in… Expand
Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 Å resolution
DNA polymerases change their specificity for nucleotide substrates with each catalytic cycle, while achieving error frequencies in the range of 10 −5to 10−6. Here we present a 2.2 Å crystal structure… Expand
DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.
A chemically modified phage T7 DNA polymerase has three properties that make it ideal for DNA sequencing by the chain-termination method. The enzyme is highly processive, catalyzing the… Expand
Jet energy scale and resolution in the CMS experiment in pp collisions at 8 TeV
Improved jet energy scale corrections, based on a data sample corresponding to an integrated luminosity of 19.7 fb^(-1) collected by the CMS experiment in proton-proton collisions at a center-of-mass… Expand
Performance of photon reconstruction and identification with the CMS detector in proton-proton collisions at s = 8 TeV
A description is provided of the performance of the CMS detector for photon reconstruction and identification in proton-proton collisions at a centre-of-mass energy of 8 TeV at the CERN LHC. Details… Expand
A single residue in DNA polymerases of the Escherichia coli DNA polymerase I family is critical for distinguishing between deoxy- and dideoxyribonucleotides.
Bacteriophage T7 DNA polymerase efficiently incorporates a chain-terminating dideoxynucleotide into DNA, in contrast to the DNA polymerases from Escherichia coli and Thermus aquaticus. The molecular… Expand
Norovirus vaccine against experimental human Norwalk Virus illness.
- R. Atmar, D. Bernstein, +8 authors Paul M. Mendelman
- The New England journal of medicine
- 7 December 2011
BACKGROUND Noroviruses cause epidemic and sporadic acute gastroenteritis. No vaccine is available to prevent norovirus illness or infection. METHODS We conducted a randomized, double-blind,… Expand
Motors, switches, and contacts in the replisome.
Replisomes are the protein assemblies that replicate DNA. They function as molecular motors to catalyze template-mediated polymerization of nucleotides, unwinding of DNA, the synthesis of RNA… Expand
Selective inactivation of the exonuclease activity of bacteriophage T7 DNA polymerase by in vitro mutagenesis.
The 3' to 5' exonuclease activity of bacteriophage T7 DNA polymerase (gene 5 protein) can be inactivated selectively by reactive oxygen species. Differences in the enzymatic properties between the… Expand