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A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.
TLDR
A coupled system that permits the exclusive expression of genes under the control of a T7 RNA polymerase promoter and its use to express high levels of phage T7 gene 5 protein, a subunit of T7 DNA polymerase is described.
Crystal structure of a bacteriophage T7 DNA replication complex at 2.2 Å resolution
TLDR
The structure of the replicative DNA polymerase from bacteriophage T7 complexed with a primer–template and a nucleoside triphosphate in the polymerase active site provides a structural basis for a metal-assisted mechanism of phosphoryl transfer by a large group of related polymerases.
DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.
TLDR
A chemically modified phage T7 DNA polymerase has three properties that make it ideal for DNA sequencing by the chain-termination method; the enzyme is highly processive, catalyzing the polymerization of thousands of nucleotides without dissociating, and processive synthesis with dITP in place of dGTP eliminates band compressions.
A single residue in DNA polymerases of the Escherichia coli DNA polymerase I family is critical for distinguishing between deoxy- and dideoxyribonucleotides.
Bacteriophage T7 DNA polymerase efficiently incorporates a chain-terminating dideoxynucleotide into DNA, in contrast to the DNA polymerases from Escherichia coli and Thermus aquaticus. The molecular
Motors, switches, and contacts in the replisome.
TLDR
The replisome of bacteriophage T7 contains a minimum of proteins, thus facilitating its study, and this review describes the molecular motors and coordination of their activities, with emphasis on the T7 replisomes.
Proteomic analysis of thioredoxin-targeted proteins in Escherichia coli.
TLDR
A comprehensive analysis of the thioredoxin-linked Escherichia coli proteome is presented by using tandem affinity purification and nanospray microcapillary tandem mass spectrometry, implicating the involvement of thiOREDoxin in at least 26 distinct cellular processes that include transcription regulation, cell division, energy transduction, and several biosynthetic pathways.
C-terminal Phenylalanine of Bacteriophage T7 Single-stranded DNA-binding Protein Is Essential for Strand Displacement Synthesis by T7 DNA Polymerase at a Nick in DNA*
TLDR
Single-stranded DNA-binding protein (gp2.5), encoded by gene 2.5 of bacteriophage T7, plays an essential role in DNA replication and enables T7 DNA polymerase to catalyze strand displacement DNA synthesis at a nick in DNA.
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