Solution conformation of the major adduct between the carcinogen (+)-anti-benzo[a]pyrene diol epoxide and DNA.
- M. Cosman, C. R. de los Santos, S. Broyde
- ChemistryProceedings of the National Academy of Sciences…
- 1 March 1992
The solution structure centered about the BP covalent adduct site in the (BP)G.C 11-mer duplex is determined by incorporating intramolecular and intermolecular proton-proton distance bounds deduced from the NMR data sets as constraints in energy minimization computations.
NMR structures of damaged DNA.
- M. Lukin, C. R. de los Santos
- ChemistryChemical Reviews
- 8 February 2006
NMR Characterization of a DNA Duplex Containing the Major Acrolein-derived Deoxyguanosine Adduct γ-OH-1,-N 2-Propano-2′-deoxyguanosine*
- C. R. de los Santos, T. Zaliznyak, F. Johnson
- Chemistry, BiologyJournal of Biological Chemistry
- 23 March 2001
It is concluded that formation of duplex DNA triggers the hydrolytic conversion of γ-OH-1,N 2-propano-2′-deoxyguanosine to an open chain form, a structure that facilitates pairing with dC during DNA replication and accounts for the surprising lack of mutagenicity associated with this DNA adduct.
Influence of benzo[a]pyrene diol epoxide chirality on solution conformations of DNA covalent adducts: the (-)-trans-anti-[BP]G.C adduct structure and comparison with the (+)-trans-anti-[BP]G.C…
- C. R. de los Santos, M. Cosman, D. Patel
- ChemistryBiochemistry
- 16 June 1992
Two-dimensional NMR techniques were applied to assign the exchangeable and non-exchangeable protons of the benzo[a]pyrenyl moiety and the nucleic acid in the modified duplex, which establish Watson-Crick base pair alignment at the [BP]G6.
Structure and stability of duplex DNA containing (5'S)-5',8-cyclo-2'-deoxyadenosine: an oxidatively generated lesion repaired by NER.
- Tatiana Zaliznyak, M. Lukin, C. R. de los Santos
- Chemistry, BiologyChemical Research in Toxicology
- 15 October 2012
Using solution state NMR spectroscopy and restrained molecular dynamics simulations, the structure of an undecameric DNA duplex having a centrally located (5'S)-5',8-cyclo-2'-deoxyadenosine residue paired to T is determined and the S-cdA·T duplex structure shows many similarities to and some intriguing differences from the recently reported structure that suggest different lesion site dynamics.
Carbon-13 nuclear magnetic resonance analysis of [1-13C]glucose metabolism in Trypanosoma cruzi. Evidence of the presence of two alanine pools and of two CO2 fixation reactions.
- B. Frydman, C. R. de los Santos, J. Cannata, J. Cazzulo
- Biology, ChemistryEuropean Journal of Biochemistry
- 1 September 1990
The non-invasive technique of 13C-nuclear magnetic resonance was applied to study glucose metabolism in vivo in Trypanosoma cruzi, the causative agent of American trypanosomiasis, and revealed the presence in T. cruzi of a reductive pathway of CO2 which is independent of the CO2 fixation reaction.
Dynamic behavior of DNA base pairs containing 8-oxoguanine.
- Xiaolin Cheng, C. Kelso, V. Hornak, C. R. de los Santos, A. Grollman, C. Simmerling
- Chemistry, BiologyJournal of the American Chemical Society
- 12 October 2005
These simulations indicate that both the dynamic and equilibrium behavior of the duplex change as a result of oxidation; these differences may provide valuable new insight into the selective action of enzymes on damaged DNA.
Integrity of duplex structures without hydrogen bonding: DNA with pyrene paired at abasic sites.
- S. Smirnov, T. Matray, E. Kool, C. R. de los Santos
- Chemistry, BiologyNucleic Acids Research
- 15 December 2002
The data indicate that both molecules adopt right-handed helical conformations with Watson- Crick alignments for all canonical base pairs, and support the idea that the stability and replication of a P.F pair is due to its ability to mimic Watson-Crick structure.
Structure and stability of DNA containing an aristolactam II-dA lesion: implications for the NER recognition of bulky adducts
- M. Lukin, T. Zaliznyak, F. Johnson, C. R. de los Santos
- Biology, ChemistryNucleic Acids Research
- 25 November 2011
NMR structural studies of an undecameric duplex damaged at its center by the presence of an ALII-dA adduct establish a locally perturbed double helical structure that accommodates the bulky adduct by displacing the counter residue into the major groove and stacking the ALII moiety between flanking bases.
Structure and stability of duplex DNA containing the 3-(deoxyguanosin-N2-yl)-2-acetylaminofluorene (dG(N2)-AAF) lesion: a bulky adduct that persists in cellular DNA.
- T. Zaliznyak, R. Bonala, F. Johnson, C. R. de los Santos
- Chemistry, BiologyChemical Research in Toxicology
- 4 May 2006
Analysis of UV melting profiles shows that the presence of dG(N(2))-AAF increases the thermal and thermodynamic stability of duplex DNA, an effect that is driven by a favorable entropy.
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