Gateway-compatible vectors for plant functional genomics and proteomics.
- K. Earley, J. Haag, C. Pikaard
- BiologyThe Plant Journal
- 1 February 2006
The utility of pEarleyGate destination vectors for the expression of epitope-tagged proteins that can be affinity captured or localized by immunofluorescence microscopy is demonstrated.
Analysis of histone acetyltransferase and histone deacetylase families of Arabidopsis thaliana suggests functional diversification of chromatin modification among multicellular eukaryotes.
- Ritu Pandey, A. Müller, R. Jorgensen
- BiologyNucleic Acids Research
- 1 December 2002
The substantial diversification of HATs and HDACs that has occurred since the divergence of plants, animals and fungi suggests a surprising degree of evolutionary plasticity and functional diversification in these core chromatin components.
Noncoding Transcription by RNA Polymerase Pol IVb/Pol V Mediates Transcriptional Silencing of Overlapping and Adjacent Genes
- A. Wierzbicki, J. Haag, C. Pikaard
- BiologyCell
- 14 November 2008
The Arabidopsis Chromatin-Modifying Nuclear siRNA Pathway Involves a Nucleolar RNA Processing Center
- O. Pontes, C. Li, C. Pikaard
- BiologyCell
- 14 July 2006
Plant Nuclear RNA Polymerase IV Mediates siRNA and DNA Methylation-Dependent Heterochromatin Formation
- Y. Onodera, J. Haag, T. Ream, Pedro Costa Nunes, O. Pontes, C. Pikaard
- Biology, ChemistryCell
- 11 March 2005
A concerted DNA methylation/histone methylation switch regulates rRNA gene dosage control and nucleolar dominance.
- Richard J. Lawrence, K. Earley, C. Pikaard
- BiologyMolecules and Cells
- 27 February 2004
An SNF2 Protein Associated with Nuclear RNA Silencing and the Spread of a Silencing Signal between Cells in Arabidopsis[W][OA]
- Lisa M. Smith, O. Pontes, D. Baulcombe
- BiologyThe Plant Cell Online
- 1 May 2007
From genetic analysis, it is concluded that another downstream part of the pathway associated with intercellular signaling requires DCL4 and at least one other protein required for 21-nucleotide trans-acting siRNAs.
An ARGONAUTE4-Containing Nuclear Processing Center Colocalized with Cajal Bodies in Arabidopsis thaliana
- C. Li, O. Pontes, S. Jacobsen
- BiologyCell
- 14 July 2006
RNA Polymerase V transcription guides ARGONAUTE4 to chromatin
- A. Wierzbicki, T. Ream, J. Haag, C. Pikaard
- BiologyNature Genetics
- 12 March 2009
The data suggest that AGO4 is guided to target loci through base-pairing of associated siRNAs with nascent Pol V transcripts, and that DEFECTIVE in MERISTEM SILENCING3, a structural maintenance of chromosomes (SMC) hinge-domain protein, functions in the assembly of Pol V transcription initiation or elongation complexes.
Erasure of histone acetylation by Arabidopsis HDA6 mediates large-scale gene silencing in nucleolar dominance.
- K. Earley, Richard J. Lawrence, C. Pikaard
- BiologyGenes & Development
- 15 May 2006
HDRA6 localizes, in part, to the nucleolus, supporting a model whereby HDA6 erases histone acetylation as a key step in an epigenetic switch mechanism that silences rRNA genes through concerted histone and DNA modifications.
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