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Slow amyloid nucleation via α-helix-rich oligomeric intermediates in short polyglutamine-containing huntingtin fragments.
The use of very short polyQ repeat lengths in htt N-terminal fragments to slow this disease-associated aggregation is described, based on a regular pattern of multimers observed in analytical ultracentrifugation, and a concentration dependence of α-helix formation in CD spectroscopy. Expand
Studying multiprotein complexes by multisignal sedimentation velocity analytical ultracentrifugation.
A computational approach for integrating multiple optical signals into the sedimentation coefficient distribution analysis of components, which combines the size-dependent hydrodynamic separation with discrimination of the extinction properties of the sedimenting species, and observes that the spectral discrimination can synergistically enhance the hydrod dynamic resolution. Expand
Model for the three‐dimensional structure of vitronectin: Predictions for the multi‐domain protein from threading and docking
The structure of vitronectin, an adhesive protein that circulates in high concentrations in human plasma, was predicted through a combination of computational methods and experimental approaches and the predictions are consistent with experimental data on purified plasma vitronECTin pertaining to protease sensitivity, ligand‐binding sites, and buried cysteines. Expand
A model for the three-dimensional structure of human plasma vitronectin from small-angle scattering measurements.
Small-angle X-ray scattering (SAXS) measurements were used to characterize vitronectin, a circulatory protein found in human plasma that functions in regulating cell adhesion and migration, as wellExpand
Plasminogen Activator Inhibitor Type 1 Promotes the Self-association of Vitronectin into Complexes Exhibiting Altered Incorporation into the Extracellular Matrix*
Evidence indicates that PAI-1 alters the adhesive functions of vitronectin by converting the protein via the higher order complex to a self-associated, multivalent species that is functionally distinct from the abundant monomeric form found in the circulation. Expand
New Insights into the Size and Stoichiometry of the Plasminogen Activator Inhibitor Type-1·Vitronectin Complex*
Competition studies using monoclonal antibodies specific for separate epitopes confirmed that the two distinct PAI-1-binding sites present on vitronectin can be occupied simultaneously, and Binding of PAi-1 to vitronECTin and association into higher order complexes is proposed to facilitate interaction with macromolecules on surfaces. Expand
Tissue‐type plasminogen activator‐plasmin‐BDNF modulate glutamate‐induced phase‐shifts of the mouse suprachiasmatic circadian clock in vitro
Together, these data are the first to demonstrate expression of these proteases in the SCN, their involvement in modulating photic phase‐shifts, and their activation of BDNF in theSCN, a potential ‘gating’ mechanism for photicphase‐resetting. Expand
Sml1p is a dimer in solution: characterization of denaturation and renaturation of recombinant Sml1p.
It appears that Sml1p is a two-domain protein where the N-terminal 8-20 residues are responsible for dimerization and the C-terminus for binding and inhibiting Rnr1p activity. Expand
Orientation of Heparin-binding Sites in Native Vitronectin
Results from this broad experimental approach argue that the behavior of the primary site is sufficient to account for the heparin binding activity of vitronectin and support an exposed orientation for the site in the structure of the native protein. Expand
Characterization of a Site on PAI-1 That Binds to Vitronectin Outside of the Somatomedin B Domain*
It is hypothesized that, together, the two sites form an extended binding area that may promote assembly of higher order vitronectin-PAI-1 complexes. Expand