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Evolution of polyketide synthases in bacteria
The emergence of resistant strains of human pathogens to current antibiotics, along with the demonstrated ability of polyketides as antimicrobial agents, provides strong motivation for understandingExpand
Approaches to identify, clone, and express symbiont bioactive metabolite genes.
This review discusses approaches to identify, clone, and express bioactive metabolite genes from symbionts of marine invertebrates, using the Bugula neritina/Endobugula sertula association as a primary example. Expand
Speciation and biosynthetic variation in four dictyoceratid sponges and their cyanobacterial symbiont, Oscillatoria spongeliae.
It is identified that at least one host switching event has occurred in this symbiosis of filamentous cyanobacterial symbiont Oscillatoria spongeliae, indicating that the chemical variation observed in these animals has a genetic foundation. Expand
Investigation of Oscillatoria spongeliae-Dominated Bacterial Communities in Four Dictyoceratid Sponges
The results demonstrate that O. spongeliae-dominated bacterial communities in different sponge species can vary considerably and increase the understanding of the bacterial communities found in marine invertebrates. Expand
New cytotoxic steroidal alkaloids from the Philippine sponge Corticium niger.
Four new steroidal alkaloids, plakinamine I-K and dihydroplakinamine K, were isolated from the marine sponge Corticium niger and elucidated by interpretation of spectroscopic data. Expand
Engineered Biosynthesis of the Antiparasitic Agent Frenolicin B and Rationally Designed Analogs in a Heterologous Host
The ability to construct a functional biosynthetic pathway to frenolicin B in the heterologous host Streptomyces coelicolor CH999, despite a lack of genetic information, illustrates the feasibility of a modular approach to engineering medicinally relevant polyketides. Expand
Cloning, sequencing, heterologous expression, and mechanistic analysis of A-74528 biosynthesis.
The complete A-74528 gene cluster, comprising 29 open reading frames, was cloned and sequenced, and shown to possess a type II polyketide synthase (PKS) at its core, and showed exceptional end-to-end sequence identity to the gene cluster responsible for biosynthesis of fredericamycin A. Expand
Total synthesis and evaluation of lamellarin α 20-Sulfate analogues
Abstract In order to explore the influence of sulfate groups on the bioactivity profiles of marine alkaloids of the lamellarin class, three such alkaloids, lamellarin α, lamellarin α 13,20-disulfateExpand
Synthesis and biological activity of novel pyranopyrones derived from engineered aromatic polyketides.
The results suggest that the diverse library of carbo- and heterocycles available through the genetic engineering of type II polyketide synthases can serve as useful building blocks to generate unique bioactive molecules. Expand
Total synthesis and evaluation of lamellarin alpha 20-Sulfate analogues.
Lamellarin H is a more potent inhibitor of HIV-1 integrase but lacked the specificity required to be medicinally useful, while lamellarin alpha 13,20-disulfate is a moderate inhibitor of both HIV- 1 integrase and cancer cell lines. Expand