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EDTA and Pb—EDTA accumulation in Brassica juncea grown in Pb—amended soil
Previous studies have shown that EDTA is necessary to solubilize soil Pb and facilitate its transport from the soil to the above ground plant tissues. These studies have also suggested that Pb is… Expand
Cloning and expression of bacterial ice nucleation genes in Escherichia coli.
- C. Orser, B. Staskawicz, N. Panopoulos, D. Dahlbeck, S. Lindow
- Biology, Medicine
- Journal of bacteriology
- 1 October 1985
Epiphytic populations of Pseudomonas syringae and Erwinia herbicola are important sources of ice nuclei that incite frost damage in agricultural crop plants. We have cloned and characterized DNA… Expand
Detection of Clavibacter michiganensis subsp. sepedonicus by DNA amplification.
Control of bacterial ring rot ultimately depends on the accurate and sensitive detection of C. michiganensis subsp. sepedonicus in infected potato tissues and tubers. Polymerase chain reaction (PCR)… Expand
Production of Se-methylselenocysteine in transgenic plants expressing selenocysteine methyltransferase
BackgroundIt has become increasingly evident that dietary Se plays a significant role in reducing the incidence of lung, colorectal and prostate cancer in humans. Different forms of Se vary in their… Expand
Purification and characterization of a tetrachloro-p-hydroquinone reductive dehalogenase from a Flavobacterium sp.
Tetrachloro-p-hydroquinone (TeCH) is the first intermediate in pentachlorophenol (PCP) degradation by Flavobacterium sp. strain ATCC 39723. We previously purified a PCP hydroxylase that oxidized PCP… Expand
Diverse substrate range of a Flavobacterium pentachlorophenol hydroxylase and reaction stoichiometries.
An understanding of the enzymatic reactions catalyzing the degradation of substituted phenols, a major group of environmental pollutants, is required for the development of biological methods for the… Expand
Unusual pattern of bacterial ice nucleation gene evolution.
- A. R. Edwards, R. A. Van Den Bussche, H. Wichman, C. Orser
- Biology, Medicine
- Molecular biology and evolution
- 1 November 1994
Bacterial ice nucleation activity (INA+ phenotype) can be traced to the product of a single gene, ina. A remarkably sparse distribution of this phenotype within three bacterial genera indicates that… Expand
Purification and properties of pentachlorophenol hydroxylase, a flavoprotein from Flavobacterium sp. strain ATCC 39723.
A pentachlorophenol (PCP) hydroxylase which catalyzed the conversion of PCP to 2,3,5,6-tetrachlorohydroquinone and released iodide from triiodophenol in the presence of NADPH and oxygen was… Expand
Confirmation of oxidative dehalogenation of pentachlorophenol by a Flavobacterium pentachlorophenol hydroxylase.
Pentachlorophenol (PCP) hydroxylase purified from Flavobacterium sp. strain ATCC 39723 converted PCP or 2,3,5,6-tetrachlorophenol to tetrachloro-p-hydroquinone (TeCH) with the co-consumption of O2… Expand
Cloning, sequence analysis, and expression of the Flavobacterium pentachlorophenol-4-monooxygenase gene in Escherichia coli.
The pcpB gene of Flavobacterium sp. strain ATCC 39723 was cloned by using a degenerate primer designed from the N-terminal sequence of the purified enzyme. The nucleotide sequence of pcpB was… Expand