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Mutation detection using a novel plant endonuclease.
TLDR
The results indicate that CEL I mutation detection is a highly sensitive method for detecting both polymorphisms and disease-causing mutations in DNA fragments as long as 1120 bp in length. Expand
Discovery of GSK1070916, a potent and selective inhibitor of Aurora B/C kinase.
TLDR
Key to the advancement of the series was the introduction of a 2-aryl group containing a basic amine onto the azaindole leading to significantly improved cellular activity leading to the identification of GSK1070916 (17k), a potent and selective ATP-competitive inhibitor of Aurora B and C. Expand
Purification, cloning, and characterization of the CEL I nuclease.
TLDR
It is proposed that CEL I exemplifies a new family of neutral pH optimum, magnesium-stimulated, mismatch duplex-recognizing nucleases, within the S1 superfamily, which includes nucleases putatively encoded by the genes BFN1 of Arabidopsis, ZEN1 of Zinnia, and DSA6 of daylily. Expand
GSK1070916, a potent Aurora B/C kinase inhibitor with broad antitumor activity in tissue culture cells and human tumor xenograft models
TLDR
Results show that GSK1070916 is a potent Aurora B/C kinase inhibitor that has the potential for antitumor activity in a wide range of human cancers. Expand
Characterization of a periplasmic S1-like nuclease coded by the Mesorhizobium loti symbiosis island.
TLDR
The biochemical characterization of M1 nuclease supports the sequence alignment that reveals the minimal 21 amino acid residues that are necessarily conserved for the structure and functions of this enzyme family and implies previously unappreciated roles of these nucleases in biological systems. Expand
Repair of aflatoxin B1 DNA adducts by the UvrABC endonuclease of Escherichia coli.
TLDR
The observation that the a flatoxin B1.N7-guanine lesion is removed rapidly, while the aflatoxin B 1-formamidopyrimidine lesion persists in the mammalian cell may have other mechanistic explanations. Expand
Discovery of a Novel 2,6-Disubstituted Glucosamine Series of Potent and Selective Hexokinase 2 Inhibitors.
TLDR
The SAR study led to the identification of potent HK2 inhibitors, such as compound 34 with greater than 100-fold selectivity over HK1, and inhibitor-bound crystal structures revealed that the HK2 enzyme could adopt an "induced-fit" conformation. Expand
Detection of simple mutations and polymorphisms in large genomic regions.
TLDR
The mutation scan correctly detected a GA insertion in the quorum-sensing regulator gene rhlR and, in addition, identified a novel intragenomic polymorphism in rrn operons, indicating very high stability of the bacterial genomes under natural non-mutator conditions. Expand
Incision at nucleotide insertions/deletions and base pair mismatches by the SP nuclease of spinach.
TLDR
It is shown that an outstanding substrate for SP is a mismatched DNA duplex, and the inability of SP to cut at guanine residues and the favoring of A-T-rich regions distinguish SP from the CEL I family of neutral pH mismatch endonucleases recently discovered in celery and other plants. Expand
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