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Molecular markers for gyrodactylids (Gyrodactylidae: Monogenea) from five fish families (Teleostei).
TLDR
Results confirm the utility of both variable region V4 and the ITS as molecular markers for Gyrodactylus species and minor differences in the sequences between Western and Eastern European populations were detected.
Species variation within the internal transcribed spacer (ITS) region of Gyrodactylus (Monogenea:Gyrodactylidae) ribosomal RNA genes.
TLDR
The ribosomal internal transcribed spacer (ITS) region from individual Gyrodactylus specimens was amplified by polymerase chain reaction (PCR) and enables rapid and clear differentiation of G. salaris, G. derjavini, and G. truttae.
Analysis of the nucleoprotein gene identifies distinct lineages of viral haemorrhagic septicaemia virus within the European marine environment.
TLDR
A ribonuclease (RNase) protection assay has been used to detect nucleotide sequence variation within the nucleoprotein gene of 39 viral haemorrhagic septicaemia virus (VHSV) isolates of European marine origin and indicated that VHSV isolates recovered from wild caught fish around the British Isles were genetically related to isolate responsible for losses in farmed turbot.
Genetic population structure of marine viral haemorrhagic septicaemia virus (VHSV).
TLDR
A marine origin of VHSV in rainbow trout aquaculture is suggested, and the capacity for molecular phylogenetic analysis to resolve complex epidemiological problems is demonstrated.
Characterization of the Infectious Salmon Anemia Virus Genomic Segment That Encodes the Putative Hemagglutinin
TLDR
The genomic segment encoding the putative hemagglutinin of infectious salmon anemia virus (ISAV) demonstrated hemadsorptive properties of the protein for salmon erythrocytes and was recognized by an ISAV-specific monoclonal antibody.
A novel variant of the infectious salmon anaemia virus (ISAV) haemagglutinin gene suggests mechanisms for virus diversity.
TLDR
A novel variant of the highly polymorphic region of the infectious salmon anaemia virus (ISAV) haemagglutinin gene was obtained from a wild adult salmon returning to a river on the East coast of Scotland and contained all motifs previously found in ISAV isolates.
Characterization of a Gyrodactylus salaris variant: infection biology, morphology and molecular genetics
TLDR
Molecular studies showed that the morphotype most likely represents a G. salaris variant, as it differed only slightly from G.salaris sensu Malmberg, 1957, which is also known to inhabit Danish watercourses.
Molecular phylogenetic analysis of the genus Gyrodactylus (Platyhelminthes: Monogenea) inferred from rDNA ITS region: subgenera versus species groups
TLDR
Characteristics of the excretory system of Gyrodactylus do not seem to be conservative enough to reveal subgenera within GyrodACTylus and it is suggested abandoning existing sub genera as indicators of phylogeny.
Development, application and validation of a Taqman real-time RT-PCR assay for the detection of infectious salmon anaemia virus (ISAV) in Atlantic salmon (Salmo salar).
TLDR
This study reports the development, validation, application and interpretation of two independent, highly sensitive and specific semi-quantitative Taqman real-time RT-PCR (qRT- PCR) methods for the detection of ISAV.
Genetic characterization of six species of diplozoids (Monogenea; Diplozoidae)
TLDR
The second internal transcribed spacer (ITS2) of the ribosomal RNA gene array of 6 species of diplozoids, E. nipponicum was the most genetically distinct species, whereas P. bliccae and P. sapae were the species most closely related.
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