Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
- D. Klionsky, K. Abdelmohsen, S. Zughaier
- 18 April 2012
There continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes, so it is important to update guidelines for monitoring autophagic activity in different organisms.
CATSPER Channel-Mediated Ca2+ Entry into Mouse Sperm Triggers a Tail-to-Head Propagation1
- Jingsheng Xia, D. Reigada, C. Mitchell, Dejian Ren
- BiologyBiology of Reproduction
- 1 September 2007
It is shown that a Ca2+ influx in the principal piece through CATSPER channels can not only initiate hyperactivated motility, but can also trigger a tail-to-head Ca2- propagation that leads to an increase in [NADH] and may regulate ATP homeostasis.
Presenilin 1 Maintains Lysosomal Ca(2+) Homeostasis via TRPML1 by Regulating vATPase-Mediated Lysosome Acidification.
- Ju-Hyun Lee, M. McBrayer, R. Nixon
- Biology, ChemistryCell Reports
- 1 September 2015
Restoration of lysosomal pH in RPE cells from cultured human and ABCA4(-/-) mice: pharmacologic approaches and functional recovery.
- Ji Liu, Wennan Lu, D. Reigada, J. Nguyen, A. Laties, C. Mitchell
- Biology, ChemistryInvestigative Ophthalmology and Visual Science
- 1 February 2008
Elevation of lysosomal pH in RPE cells interferes with the degradation of outer segments and may contribute to the pathologies associated with A2E.
A release mechanism for stored ATP in ocular ciliary epithelial cells.
- C. Mitchell, D. A. Carré, A. McGlinn, R. Stone, M. Civan
- BiologyProceedings of the National Academy of Sciences…
- 9 June 1998
Both layers of the ciliary epithelium store and release ATP, and purines likely modulate aqueous humor flow by paracrine and/or autocrine mechanisms within the two cell layers of this epithelia.
Lysosomal alkalinization, lipid oxidation, and reduced phagosome clearance triggered by activation of the P2X7 receptor
- Sonia Guha, Gabriel C. Baltazar, C. Mitchell
- BiologyThe FASEB Journal
- 1 November 2013
It is demonstrated that stimulation of the P2X7 receptor for ATP alkalinizes lysosomes in cultured human retinal pigmented epithelial (RPE) cells and impairs lysOSomal function, suggesting a possible role for overstimulation in diseases of accumulation.
Pannexin 1 channels mediate the release of ATP into the lumen of the rat urinary bladder
- J. Beckel, S. Daugherty, W. C. Groat
- Biology, MedicineJournal of Physiology
- 15 April 2015
The data indicate a role for pannexin‐mediated luminal ATP release in both the physiological and pathophysiological control of micturition and suggest that urothelialpannexin may be a viable target for the treatment of overactive bladder disorders.
Elevated pressure triggers a physiological release of ATP from the retina: Possible role for pannexin hemichannels
- D. Reigada, W. Lu, M. Zhang, C. Mitchell
- 19 November 2008
Release of ATP by a human retinal pigment epithelial cell line: potential for autocrine stimulation through subretinal space
- C. Mitchell
- BiologyJournal of Physiology
- 1 July 2001
The results suggest that growth factors, pyrimidines and changes in tonicity could trigger ATP release into subretinal space and may be capable of autocrine stimulation of ATP receptors, while conversion to adenosine by ecto‐enzymes could alter phagocytosis.
Identification of the A3 adenosine receptor in rat retinal ganglion cells.
- Mei Zhang, M. Budak, C. Mitchell
- BiologyMolecular Vision
- 1 May 2005
Retinal ganglion cells express A3 adenosine receptor mRNA and Stimulation of this receptor can reduce the Ca2+ overload following excessive activation of P2X7 receptors.