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Isolation of secretory cells from plant glandular trichomes and their use in biosynthetic studies of monoterpenes and other gland products.
A new procedure was developed for isolating large numbers of highly purified secretory cells and cell-free preparations derived from them, which provide useful starting materials for the purification of several key enzymes of monoterpene metabolism.
Monoterpene biosynthesis: specificity of the hydroxylations of (-)-limonene by enzyme preparations from peppermint (Mentha piperita), spearmint (Mentha spicata), and perilla (Perilla frutescens)
Microsomal preparations from the epidermal oil glands of Mentha piperita, Mentha spicata, and Perilla frutescens leaves catalyze the NADPH- and O2-dependent allylic hydroxylation of the monoterpene
Binary Insecticidal Crystal Protein from Bacillus thuringiensis, Strain PS149B1: Effects of Individual Protein Components and Mixtures in Laboratory Bioassays
Results did not support the formation of a stable, fixed-ratio complex of the two component proteins, although both proteins are clearly required for maximal insecticidal activity.
Guidelines for the Validation and Use of Immunoassays for Determination of Introduced Proteins in Biotechnology Enhanced Crops and Derived Food Ingredients
This collaborative effort represents the combined expertise of several organizations to reach consensus on establishing guidelines for the validation and use of immunoassay methods for agricultural biotechnology applications.
Determination of spinosad and its metabolites in food and environmental matrices. 3. Immunoassay methods.
Residue methods utilizing a magnetic particle-based immunoassay (IA) test kit have been developed and validated for determining spinosad in environmental and food matrices.
Growth pattern and carbon allocation to volatile leaf terpenes under nitrogen-limiting conditions in Heterotheca subaxillaris (Asteraceae)
Higher levels of carbon-based herbivore-deterring chemicals in nitrate-limited plants may increase net productivity through retention of nitrogen that would otherwise be lost to herbivory.
Changes in leaf mono- and sesquiterpene metabolism with nitrate availability and leaf age inHeterotheca subaxillaris
The results suggest that the higher leaf volatile terpene content observed under nitrate limitation apparently results from increased synthesis per leaf and accumulation of mono- and sesquiterpenes in immature leaves of nitratepoor plants.
Rapid Degradation of Cry1F Delta-Endotoxin in Soil
Based on the decrease in toxicity over time, the half-life of the microbial Cry1F delta-endotoxin was estimated to be less than 1 d under laboratory conditions, indicating a rapid decay rate in soil.
Practical immunochemical method for determination of 3,5, 6-trichloro-2-pyridinol in human urine: applications and considerations for exposure assessment.
Residues of 3,5,6-TCP determined using both immunochemical and gas chromatography with mass spectrometric detection correlate well.
Development of a Magnetic Particle-Based Enzyme Immunoassay for the Quantitation of Chlorpyrifos in Water
A competitive enzyme immunoassay for the quantitation of chlorpyrifos in water that utilizes amine-terminated superparamagnetic particles as the solid phase to covalently attach mouse monoclonal