Growth independent rhamnolipid production from glucose using the non-pathogenic Pseudomonas putida KT2440
- A. Wittgens, T. Tiso, L. Blank
- Biology, EngineeringMicrobial Cell Factories
- 17 October 2011
The metabolic engineering of a rhamnolipid producing Pseudomonas putida KT2440, a strain certified as safety strain using glucose as carbon source to avoid cumbersome product purification, is reported, finding a functional alternative to the pathogen P. aeruginosa.
The baffled microtiter plate: Increased oxygen transfer and improved online monitoring in small scale fermentations
- M. Funke, Sylvia Diederichs, F. Kensy, C. Müller, J. Büchs
- EngineeringBiotechnology and Bioengineering
- 15 August 2009
This study investigates how baffling and the well geometry affect the maximum oxygen transfer capacity (OTRmax) in microtiter plates and shows a six‐petal flower‐shaped well geometry was shown to be the optimal solution taking into account all aforementioned criteria.
Microfluidic biolector—microfluidic bioprocess control in microtiter plates
- M. Funke, A. Buchenauer, J. Büchs
- Engineering, BiologyBiotechnology and Bioengineering
- 15 October 2010
The novel concept of a microfermentation system described in this work combines a fiber‐optic online‐monitoring device for microtiter plates (MTPs)—the BioLector technology—together with microfluidic control of cultivation processes in volumes below 1 mL with applications in fermentations of Escherichia coli under pH‐controlled and fed‐batch conditions in shaken MTPs.
Respiration activity monitoring system (RAMOS), an efficient tool to study the influence of the oxygen transfer rate on the synthesis of lipopeptide by Bacillus subtilis ATCC6633.
- J. Guez, C. Müller, P. Danzé, J. Büchs, P. Jacques
- BiologyJournal of Biotechnology
- 20 March 2008
Robo-Lector – a novel platform for automated high-throughput cultivations in microtiter plates with high information content
The Robo-Lector generates extensive kinetic data in high-throughput cultivations, particularly for biomass and fluorescence protein formation, and can be combined with small-scale downstream processing techniques and analytical assays.
Bioprocess Control in Microscale: Scalable Fermentations in Disposable and User-Friendly Microfluidic Systems
- M. Funke, A. Buchenauer, J. Büchs
- Engineering, BiologyMicrobial Cell Factories
- 13 November 2010
A user-friendly and disposable microfluidic system could be established which allows scaleable, fully controlled and fully monitored fermentations in working volumes below 1 milliliter.
Application of an improved continuous parallel shaken bioreactor system for three microbial model systems
- A. Akgün, C. Müller, Ramona Engmann, J. Büchs
- Biology, EngineeringBioprocess and biosystems engineering (Print)
- 4 January 2008
A continuous parallel shaken bioreactor system was specifically manufactured from quartz glass and provides a geometric accuracy of <1 mm and many more reading points were obtained with the parallel shakes in the same time at much lower consumption of culture media.
Increased product formation induced by a directed secondary substrate limitation in a batch Hansenula polymorpha culture
- Kirsten Kottmeier, C. Müller, R. Huber, J. Büchs
- Biology, EngineeringApplied Microbiology and Biotechnology
- 1 March 2010
To study the impact of directed limitations caused by various secondary substrates on the growth and product formation of the methylotrophic yeast Hansenula polymorpha, the cultivation systems respiration activity monitoring system (RAMOS) and BioLector were used in parallel.
Design and validation of a parallelized micro‐photobioreactor enabling phototrophic bioprocess development at elevated throughput
- H. Morschett, Danny Schiprowski, M. Oldiges
- Biology, EngineeringBiotechnology and Bioengineering
- 1 January 2017
A microtiter plate based micro‐photobioreactor system was developed enabling 48‐fold parallelized cultivation and significantly contributes to improving the time efficiency during the development of phototrophic bioprocesses.
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