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Activation and identification of five clusters for secondary metabolites in Streptomyces albus J1074
A cluster for glycosylated compounds (paulomycins) was also identified by comparison of the high‐performance liquid chromatography profiles of the wild‐type strain with that of a mutant in which two key enzymes of the cluster were simultaneously deleted. Expand
Minimum Information about a Biosynthetic Gene cluster.
This work proposes the Minimum Information about a Biosynthetic Gene cluster (MIBiG) data standard, to facilitate consistent and systematic deposition and retrieval of data on biosynthetic gene clusters. Expand
Two glycosyltransferases and a glycosidase are involved in oleandomycin modification during its biosynthesis by Streptomyces antibioticus
  • L. Quirós, Ignacio Aguirrezabalaga, Carlos Olano, C. Méndez, José A. Salas
  • Biology, Medicine
  • Molecular microbiology
  • 1 June 1998
A model is proposed integrating these and previously reported results for intracellular inactivation, secretion and extracellular reactivation of oleandomycin, with OleI being much more specific for ole fandomycin. Expand
The biosynthetic gene cluster for the antitumor rebeccamycin: characterization and generation of indolocarbazole derivatives.
The cloned genes should help to elucidate the molecular basis for indolocarbazole biosynthesis and set the stage for the generation of novel indolo-rebeccamycin analogues by genetic engineering. Expand
A regioselective tryptophan 5-halogenase is involved in pyrroindomycin biosynthesis in Streptomyces rugosporus LL-42D005.
The halogenase gene could be overexpressed in Pseudomonas fluorescens BL915 DeltaORF1 and was purified to homogeneity by immobilized metal chelate ion affinity chromatography. Expand
Improving production of bioactive secondary metabolites in actinomycetes by metabolic engineering.
In this review, the development of recombinant DNA technology has provided new tools for approaching yields improvement by means of genetic manipulation of biosynthetic pathways as applied to bioactive secondary metabolites produced by actinomycetes. Expand
Antitumor Compounds from Marine Actinomycetes
Actinomycetes are the producers of a large number of natural products with different biological activities, including antitumor properties, and have attracted special attention in the last years for their ability to produce interesting pharmacological lead compounds. Expand
Reevaluation of the Violacein Biosynthetic Pathway and its Relationship to Indolocarbazole Biosynthesis
The violacein pathway is studied through expression of vio genes in Escherichia coli and Streptomyces albus and a pair of genes (vioAB) was functionally equivalent to the homologous pair in the indolocarbazole pathway (rebOD), directing the formation of chromopyrrolic acid. Expand
Combinatorial biosynthesis of antitumor indolocarbazole compounds.
A biological process based on combinatorial biosynthesis for the production of indolocarbazole compounds (or their precursors) in engineered microorganisms as a complementary approach to chemical synthesis is reported. Expand
The aureolic acid family of antitumor compounds: structure, mode of action, biosynthesis, and novel derivatives
Members of the aureolic acid family are tricyclic polyketides with antitumor activity which are produced by different streptomycete species and have been studied in detail by gene sequencing, insertional inactivation, and gene expression. Expand