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16S rRNA-based assays for quantitative detection of universal, human-, cow-, and dog-specific fecal Bacteroidales: a Bayesian approach.
TLDR
The design and validation of new TaqMan((R)) assays for microbial source tracking based on the amplification of fecal 16S rRNA marker sequences from uncultured cells of the order Bacteroidales were reported, showing the new methodologies to be more specific and sensitive. Expand
Application of Real-Time PCR To Study Effects of Ammonium on Population Size of Ammonia-Oxidizing Bacteria in Soil
TLDR
A quantitative real-time PCR assay targeting part of the ammonia-monooxygenase gene (amoA) was developed to estimate AOB population size in soil and found that the population size was significantly greater in annually fertilized versus unfertilized soil, suggesting a long-term effect of ammonium fertilization on AOBpopulation size. Expand
Concentrations of a Koi herpesvirus (KHV) in tissues of experimentally infected Cyprinus carpio koi as assessed by real-time TaqMan PCR.
TLDR
Although there were no recognized patterns to viral DNA concentrations as found in different tissues over time, KHV genome copies for all tissues increased with time post virus exposure and with water temperature. Expand
Feline infectious peritonitis: Role of the feline coronavirus 3c gene in intestinal tropism and pathogenicity based upon isolates from resident and adopted shelter cats
TLDR
No evidence could be found to support a recent theory that FIPVs and FECVs are genetically distinct, and an intact 3c appears to be essential for intestinal replication. Expand
Evidence of the Human Granulocytic Ehrlichiosis Agent in Ixodes ricinus Ticks in Switzerland
TLDR
The occurrence of this agent in I. ricinus in Switzerland presents a potential danger of transmission of granulocytic ehrlichiosis to dogs, horses, and humans. Expand
Viruses in diarrhoeic dogs include novel kobuviruses and sapoviruses.
TLDR
Canine kobuvirus, the first sequenced canine picornavirus and the closest genetic relative of the diarrhoea-causing human Aichi virus, was detected at high frequency in the faeces of both healthy and diarrhoeic dogs, highlighting the high frequency of new virus detection possible even in extensively studied animal species using metagenomics approaches. Expand
Real-time RT-PCR (TaqMan) assays for the detection of Grapevine Leafroll associated viruses 1-5 and 9.
TLDR
TaqMan RT-PCR was more sensitive than conventional one-step RT- PCR for testing different isolates of these viruses either using RNA or crude tissue extract and showed that when using total RNA extracted either by the Qiagen RNeasy method or by an ABI automated system more isolates were detected. Expand
Detection of Toxoplasma gondii-like oocysts in cat feces and estimates of the environmental oocyst burden.
TLDR
Despite the low prevalence and short duration of T gondii oocyst shedding by cats detected in the present and former surveys, the sheer numbers of oocysts shed by cats during initial infection could lead to substantial environmental contamination. Expand
Quantitation of bovine cytokine mRNA in milk cells of healthy cattle by real-time TaqMan polymerase chain reaction.
TLDR
A novel methodology to quantitate bovine cytokines and growth factors contributing to immunity against bacterial infections of the mammary gland in cattle by decreasing the numbers of working steps shortened the time for analysis and increased accuracy. Expand
Circovirus in Tissues of Dogs with Vasculitis and Hemorrhage
TLDR
The results indicate that circovirus, alone or in co-infection with other pathogens, might contribute to illness and death in dogs. Expand
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