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A sensitive new substrate for chymotrypsin.
TLDR
A new substrate for chymotrypsin, succinyl-Ala—Ala —Pro—Phe-p-nitroanilide has been synthesized and is readily soluble and stable to hydrolysis in Tris buffer at pH 8.0. Expand
Pbx proteins display hexapeptide-dependent cooperative DNA binding with a subset of Hox proteins.
TLDR
It is demonstrated that interactions between Hox and Pbx proteins modulate their DNA-binding properties, suggesting that PbX and Hox proteins act in parallel as heterotypic complexes to regulate expression of specific subordinate genes. Expand
AbdB-like Hox proteins stabilize DNA binding by the Meis1 homeodomain proteins
TLDR
The Hoxa-9 gene is frequently activated by viral integration in the same BXH-2 leukemias, suggesting a biological synergy between these two distinct classes of homeodomain proteins in causing malignant transformation. Expand
Overexpression of HOXB4 in hematopoietic cells causes the selective expansion of more primitive populations in vitro and in vivo.
TLDR
Serial transplantation studies revealed a greatly enhanced ability of HOXB4-transduced bone marrow cells to regenerate the most primitive hematopoietic stem cell compartment resulting in 50-fold higher numbers of transplantable totipotent hematoplastic stem cells in primary and secondary recipients, compared with serially passaged neo-infected control cells. Expand
HOXA9 Forms Triple Complexes with PBX2 and MEIS1 in Myeloid Cells
TLDR
Taken together, these data suggest that in myeloid leukemia cells MEIS1 forms trimeric complexes with PBX and HOXA9, which in turn can bind to consensus PBX-HOXA 9 DNA targets. Expand
Mice bearing a targeted interruption of the homeobox gene HOXA9 have defects in myeloid, erythroid, and lymphoid hematopoiesis.
TLDR
A physiologic role for a HOX gene in blood cell differentiation is demonstrated, with the greatest apparent influence of HOXA9 at the level of the committed progenitor. Expand
Pbx modulation of Hox homeodomain amino-terminal arms establishes different DNA-binding specificities across the Hox locus
TLDR
Interactions with Pbx were found to play an essential role in modulating Hox homeodomain amino-terminal arm contact with DNA in the core of the Hox half site such that heterodimers of different compositions could distinguish single nucleotide alterations in the H Cox half site both in vitro and in cellular assays measuring transactivation. Expand
The Abd-B-like Hox Homeodomain Proteins Can Be Subdivided by the Ability to Form Complexes with Pbx1a on a Novel DNA Target*
TLDR
The data suggest that Abd-B-like Hox proteins bind to a novel TTAC site and can be divided by their cooperative binding to DNA with Pbx1a. Expand
Differential expression of homeobox genes in functionally distinct CD34+ subpopulations of human bone marrow cells.
TLDR
The expression of a broad range of Hox genes in primitive hematopoietic cells are demonstrated and point to the existence of a regulated program of H Cox gene expression during their normal development. Expand
Loss of expression of the Hoxa-9 homeobox gene impairs the proliferation and repopulating ability of hematopoietic stem cells.
TLDR
Hoxa-9 appears to be specifically required for normal hematopoietic stem cell function both in vitro and in vivo, and Retroviral transduction with a HoxA-9 expression vector dramatically enhanced the cytokine-driven proliferation and in vitro engraftment of Hoxi-9-/- marrow cells. Expand
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