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Structural basis for the activation of anthrax adenylyl cyclase exotoxin by calmodulin
TLDR
Allosteric changes of oedema factor provide the first molecular details of how calmodulin modulates one of its targets. Expand
Adaptive Licensing: Taking the Next Step in the Evolution of Drug Approval
TLDR
This article summarizes recent adaptive licensing proposals; discusses how proposals might be translated into practice, with illustrations in different therapeutic areas; and identifies unresolved issues to inform decisions on the design and implementation of AL. Expand
An Extended Conformation of Calmodulin Induces Interactions between the Structural Domains of Adenylyl Cyclase from Bacillus anthracis to Promote Catalysis*
TLDR
A genetic screen probing loss-of-function mutations of EF3 and site-directed mutations based on the homology of the edema factor family revealed a conserved pair of aspartate residues and an arginine that are important for catalysis. Expand
Differences in the binding sites of two site-3 sodium channel toxins
TLDR
This work examines the interaction of chimeric channels with a form of ApB in which the two nonconserved basic residues, Arg-12 and Lys-49, were converted to the corresponding neutral amino acids from ApA and concludes that the differential component of ApA binding is controlled by domain 4 and that some component ofApB binding is not shared by ApA. Expand
Component-Specific Analysis of Plasma Protein Corona Formation on Gold Nanoparticles Using Multiplexed Surface Plasmon Resonance.
TLDR
Dramatic differences in k(on) for individual components are shown as primary determinants of protein affinities, with k( on) ranging over nearly two orders of magnitude for the proteins studied, while k(off) remains within a factor of two for the set. Expand
In vivo prevention of arterial restenosis with paclitaxel-encapsulated targeted lipid–polymeric nanoparticles
TLDR
A systemically administered, targeted NP system to deliver an antiproliferative agent to injured vasculature and an ∼50% reduction in arterial stenosis was observed with targeted NP treatment, which could potentially provide a safe and efficacious option in the management of CAD. Expand
Multiple cationic residues of anthopleurin B that determine high affinity and channel isoform discrimination.
TLDR
The role of cationic residues is investigated by characterizing toxin mutants in which two such residues are replaced simultaneously, which reduced affinity of the toxin for neuronal channels to a much greater extent than for cardiac channels and produced affinities only slightly lower than for ApA in each case. Expand
Crystallization and preliminary X-ray study of the edema factor exotoxin adenylyl cyclase domain from Bacillus anthracis in the presence of its activator, calmodulin.
TLDR
The crystallization and initial experimental phasing of crystals containing a complex of edema factor adenylyl cyclase domain and calmodulin are reported here and the self-rotation function solution suggests three edema factors-calmodulin complexes in each asymmetric unit. Expand
Structure of anthrax edema factor-calmodulin-adenosine 5'-(alpha,beta-methylene)-triphosphate complex reveals an alternative mode of ATP binding to the catalytic site.
TLDR
This structure of the catalytic domain of EF (EF3) in complex with calmodulin and adenosine 5'-(alpha,beta-methylene)-triphosphate (AMPCPP) is reported, with K382 and F586 should play key roles in the recognition of adenine. Expand
The Importance of Sex Stratification in Autoimmune Disease Biomarker Research: A Systematic Review
TLDR
The practice of sex stratification in biomarker research may not only advance the discovery of sex-specific AD biomarkers but more importantly, promote reproducibility in subsequent validation studies, thus easing the translation of these novel biomarkers from bench to bedside to improve AD diagnosis. Expand
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