• Publications
  • Influence
A Disulfide Relay System in the Intermembrane Space of Mitochondria that Mediates Protein Import
TLDR
It is suggested that the existence of a disulfide exchange system in the IMS is unexpected in view of the free exchange of metabolites between IMS and cytosol via porin channels, and reflects the evolutionary origin of the I MS from the periplasmic space of the prokaryotic ancestors of mitochondria. Expand
The J domain–related cochaperone Tim16 is a constituent of the mitochondrial TIM23 preprotein translocase
TLDR
This work has identified a novel essential cochaperone, Tim16, related to J-domain proteins and forms a stable subcomplex with the J protein Tim14, which is a constituent of the TIM23 preprotein translocase, where it exerts crucial functions in the import motor. Expand
Tim50, a novel component of the TIM23 preprotein translocase of mitochondria
TLDR
It is suggested that Tim50 plays a crucial role in the transfer of preproteins from the TOM complex to the TIM23 complex through the intermembrane space. Expand
Integrative Analysis of the Mitochondrial Proteome in Yeast
TLDR
It is shown that a combination of complementary approaches like deletion phenotype screening and mass spectrometry can identify over 75% of the known mitochondrial proteome, which has implications for choosing optimal genome-wide approaches for the study of other cellular systems, including organelles and pathways in various species. Expand
Mia40, a novel factor for protein import into the intermembrane space of mitochondria is able to bind metal ions
TLDR
It is concluded that Mia40 is the first essential component of a specific translocation pathway of metal‐binding IMS proteins that is able to bind zinc and copper ions. Expand
Structural and Functional Roles of the Conserved Cysteine Residues of the Redox-regulated Import Receptor Mia40 in the Intermembrane Space of Mitochondria*
TLDR
It is concluded that the cysteine residues of the twin CX9C motif have a structural role and stabilize Mia40, and the disulfide bond formed by the third and sixth cysteines apparently supports a conformation crucial for the function of Mia40. Expand
Selective inhibitors of the FK506-binding protein 51 by induced fit.
TLDR
It is demonstrated that selective inhibition of FKBP51 enhances neurite elongation in neuronal cultures and improves neuroendocrine feedback and stress-coping behavior in mice, providing the structural and functional basis for the development of mechanistically new antidepressants. Expand
Large FK506-Binding Proteins Shape the Pharmacology of Rapamycin
TLDR
It is shown that rapamycin complexes of larger FKBP family members can tightly bind to mTOR and potently inhibit its kinase activity, providing a mechanistic rationale for preferential mTOR inhibition in specific cell or tissue types by engaging specific FK BP homologs. Expand
A C-terminal HSP90 inhibitor restores glucocorticoid sensitivity and relieves a mouse allograft model of Cushing disease
TLDR
It is shown that corticotroph adenomas overexpress HSP90 compared to the normal pituitary, which suggests that the pathogenesis of Cushing disease caused by overexpression of heat shock proteins and consequently misregulated GR sensitivity may be overcome pharmacologically with an appropriate H SP90 inhibitor. Expand
Reconstituted TOM core complex and Tim9/Tim10 complex of mitochondria are sufficient for translocation of the ADP/ATP carrier across membranes.
TLDR
The patterns of binding sites on the substrate proteins suggest a mechanism by which portions of membrane-spanning segments together with flanking hydrophilic segments are recognized and bound by the Tim9/Tim10 complex as they emerge from the TOM complex into the intermembrane space. Expand
...
1
2
3
4
5
...