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Structure of cyclodextrin glycosyltransferase refined at 2.0 A resolution.
The previously reported structural model of cyclodextrin glycosyltransferase (EC from Bacillus circulans has been improved. For this purpose the known sequence was built into an electronExpand
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Catalytic center of cyclodextrin glycosyltransferase derived from X-ray structure analysis combined with site-directed mutagenesis.
An X-ray structure analysis of a crystal of mutant Asp229----Ala of cyclodextrin glycosyltransferase from Bacillus circulans (Ec that had been shortly exposed to beta-cyclodextrin showedExpand
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A Simple Procedure for Selective Hydroxylation of L-Proline and L-Pipecolic Acid with Recombinantly Expressed Proline Hydroxylases
Due to their diverse regio- and stereoselectivities, proline hydroxylases provide a straightforward access to hydroxprolines and other hydroxylated cylic amino acids, valuable chiral building blocksExpand
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Advances in Bispecific Antibodies Engineering: Novel Concepts for Immunotherapies
Bispecific antibodies are emerging as novel approach for immunotherapies by combining two antigen-recognizing elements into a single construct that is able to simultaneously bind to two distinctExpand
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Engineering a heavy atom derivative for the X-ray structure analysis of cyclodextrin glycosyltransferase.
Based on a preliminary structural model of cyclodextrin glycosyltransferase from Bacillus circulans (EC, Ser428 and Ser475 of the enzyme were mutated to cysteines in order to produceExpand
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Fluorescence Polarisation Immunoassay
Since the introduction of radio immunoassay techniques [1], many different assay principles based on antibodies as specifiers have become routine tools in clinical diagnostics. HeterogeneousExpand
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Generation and characterisation of monoclonal antibodies to oleanolic acid.
Monoclonal antibodies to oleanolic acid, a pentacyclic triterpene, were generated using the hybridoma fusion method described by Kohler and Milstein . Protein conjugates of the target molecule forExpand
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Tertiary alcohol preferred: Hydroxylation of trans-3-methyl-L-proline with proline hydroxylases
The enzymatic synthesis of tertiary alcohols by the stereospecific oxidation of tertiary alkyl centers is a most-straightforward but challenging approach, since these positions are stericallyExpand
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Development of bispecific molecules for the in situ detection of protein-protein interactions and protein phosphorylation.
Investigation of protein-protein interactions (PPIs) and protein phosphorylation in clinical tissue samples can offer valuable information about the activation status and function of proteinsExpand
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