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Structure of cyclodextrin glycosyltransferase refined at 2.0 A resolution.
The previously reported structural model of cyclodextrin glycosyltransferase (EC 22.214.171.124) from Bacillus circulans has been improved. For this purpose the known sequence was built into an electron… Expand
Catalytic center of cyclodextrin glycosyltransferase derived from X-ray structure analysis combined with site-directed mutagenesis.
An X-ray structure analysis of a crystal of mutant Asp229----Ala of cyclodextrin glycosyltransferase from Bacillus circulans (Ec 126.96.36.199) that had been shortly exposed to beta-cyclodextrin showed… Expand
A Simple Procedure for Selective Hydroxylation of L-Proline and L-Pipecolic Acid with Recombinantly Expressed Proline Hydroxylases
Due to their diverse regio- and stereoselectivities, proline hydroxylases provide a straightforward access to hydroxprolines and other hydroxylated cylic amino acids, valuable chiral building blocks… Expand
Advances in Bispecific Antibodies Engineering: Novel Concepts for Immunotherapies
Bispecific antibodies are emerging as novel approach for immunotherapies by combining two antigen-recognizing elements into a single construct that is able to simultaneously bind to two distinct… Expand
STRUCTURE OF CYCLODEXTRIN GLYCOSYLTRANSFERASE REFINED AT 2.0 ANGSTROMS RESOLUTION
Engineering a heavy atom derivative for the X-ray structure analysis of cyclodextrin glycosyltransferase.
Based on a preliminary structural model of cyclodextrin glycosyltransferase from Bacillus circulans (EC 188.8.131.52), Ser428 and Ser475 of the enzyme were mutated to cysteines in order to produce… Expand
Fluorescence Polarisation Immunoassay
Since the introduction of radio immunoassay techniques , many different assay principles based on antibodies as specifiers have become routine tools in clinical diagnostics. Heterogeneous… Expand
Generation and characterisation of monoclonal antibodies to oleanolic acid.
- K. Brand, C. Klein, I. Zündorf, T. Dingermann, W. Knoess
- Biology, Medicine
- Planta medica
- 1 October 2004
Monoclonal antibodies to oleanolic acid, a pentacyclic triterpene, were generated using the hybridoma fusion method described by Kohler and Milstein . Protein conjugates of the target molecule for… Expand
Tertiary alcohol preferred: Hydroxylation of trans-3-methyl-L-proline with proline hydroxylases
The enzymatic synthesis of tertiary alcohols by the stereospecific oxidation of tertiary alkyl centers is a most-straightforward but challenging approach, since these positions are sterically… Expand
Development of bispecific molecules for the in situ detection of protein-protein interactions and protein phosphorylation.
- Jan van Dieck, Volker Schmid, +17 authors M. Tacke
- Medicine, Chemistry
- Chemistry & biology
- 20 March 2014
Investigation of protein-protein interactions (PPIs) and protein phosphorylation in clinical tissue samples can offer valuable information about the activation status and function of proteins… Expand