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A versatile toolbox for PCR‐based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes
Using the provided cassettes for N‐ and C‐terminal gene tagging or for deletion of any given gene, a set of only four primers is required, which makes this method very cost‐effective and reproducible. Expand
Evidence that the Ipl1-Sli15 (Aurora Kinase-INCENP) Complex Promotes Chromosome Bi-orientation by Altering Kinetochore-Spindle Pole Connections
IPL1 function in cells that cannot replicate their chromosomes but nevertheless duplicate their spindle pole bodies (SPBs) is investigated and the possibility that Ipl1-Sli15 facilitates bi-orientation by promoting turnover of kinetochore-SPB connections until traction of sister Kinetochores toward opposite spindle poles creates tension in the surrounding chromatin is raised. Expand
Post-translational regulation of the microtubule cytoskeleton: mechanisms and functions
Functional studies demonstrating that post-translational modifications may regulate microtubule functions through an amazing range of mechanisms are demonstrated. Expand
CLIP-170 tracks growing microtubule ends by dynamically recognizing composite EB1/tubulin-binding sites
Re reconstitute a vertebrate plus end tracking system composed of the most prominent +TIPs, end-binding protein 1 (EB1) and CLIP-170, in vitro and dissect their end-tracking mechanism, finding that EB1 autonomously recognizes specific binding sites present at growing microtubule ends. Expand
A Family of Protein-Deglutamylating Enzymes Associated with Neurodegeneration
The results reveal that controlling the length of the polyglutamate side chains on tubulin is critical for neuronal survival and is directly linked to neurodegeneration. Expand
The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control
Although conditional lethal spc24‐2 and spc25‐7 cells form a mitotic spindle, the kinetochores remain in the mother cell body and fail to segregate the chromosomes, indicating that Spc24p has a function in checkpoint control. Expand
The tubulin code: Molecular components, readout mechanisms, and functions
  • C. Janke
  • Biology, Medicine
  • The Journal of cell biology
  • 18 August 2014
This work has shed light on how posttranslational modifications of tubulin could contribute to a “tubulin code” that coordinates the complex functions of microtubules in cells. Expand
Tubulin post-translational modifications: encoding functions on the neuronal microtubule cytoskeleton
Recent studies are discussed in relation to how the combinatorial use of tubulin modifications could generate a dynamic microtubule code, and how such a code might regulate basic as well as higher-order neuronal functions. Expand
Tubulin polyglutamylation stimulates spastin-mediated microtubule severing
Microtubules with long polyglutamylated C-terminal tails are more prone to severing by spastin, establishing the importance of tubulin posttranslational modifications.
A targeted multienzyme mechanism for selective microtubule polyglutamylation.
A multienzyme mechanism of polyglutamylation is proposed that can explain how the diversity ofpolyglutamate side chains of variable lengths on selected types of MTs is controlled at the molecular level. Expand