The ins(ide) and out(side) of dolichyl phosphate biosynthesis and recycling in the endoplasmic reticulum.
- B. Schenk, F. Fernández, C. J. Waechter
- BiologyGlycobiology
- 1 May 2001
The precursor oligosaccharide donor for protein N-glycosylation in eukaryotes, Glc3Man9GlcNAc(2)-P-P-dolichol, is synthesized in two stages on both leaflets of the rough endoplasmic reticulum (ER).…
Nogo‐B receptor is necessary for cellular dolichol biosynthesis and protein N‐glycosylation
- Kenneth D. Harrison, E. Park, W. Sessa
- Biology, ChemistryEMBO Journal
- 15 June 2011
Identification of Nogo‐B receptor (NgBR) as an essential component of the cis‐IPTase machinery yields insights into the regulation of dolichol biosynthesis.
Evidence That the wzxE Gene of Escherichia coli K-12 Encodes a Protein Involved in the Transbilayer Movement of a Trisaccharide-Lipid Intermediate in the Assembly of Enterobacterial Common Antigen*
- P. D. Rick, K. Barr, K. Sankaran, Junko Kajimura, J. Rush, C. J. Waechter
- BiologyJournal of Biological Chemistry
- 9 May 2003
Results support the conclusion that thewzxE gene encodes a membrane protein involved in the transbilayer movement of lipid III in E. coli.
Biosynthesis of Mycobacterial Lipoarabinomannan*
- G. Besra, C. Morehouse, C. Rittner, C. J. Waechter, P. Brennan
- BiologyJournal of Biological Chemistry
- 18 July 1997
A novel form of cell-free incubation involving in vitro and in situ labeling with GDP-[14C]Man of the polyprenyl-P-mannoses and the simpler PIMs of mycobacterial membranes demonstrated the synthesis of a novel α(1→6)-linked linear form of LM at the expense of the C35/C50-P -Man.
Deficiency of UDP‐GlcNAc:Dolichol Phosphate N‐Acetylglucosamine‐1 Phosphate Transferase (DPAGT1) Causes a Novel Congenital Disorder of Glycosylation Type Ij
- Xiaohuai Wu, J. Rush, H. Freeze
- BiologyHuman Mutation
- 1 August 2003
A novel CDG type, CDG‐Ij, is identified, resulting from deficiency in UDP‐GlcNAc: dolichol phosphate N‐acetyl‐glucosamine‐1 phosphate transferase (GPT) activity encoded by DPAGT1, which is concluded to be responsible for the CDG symptoms in this patient.
Novel salvage pathway utilizing farnesol and geranylgeraniol for protein isoprenylation.
- D. Crick, D. Andres, C. J. Waechter
- BiologyBiochemical and Biophysical Research…
- 28 August 1997
Requirement of the Lec35 gene for all known classes of monosaccharide-P-dolichol-dependent glycosyltransferase reactions in mammals.
- M. Anand, J. Rush, M. Lehrman
- Biology, ChemistryMolecular Biology of the Cell
- 1 February 2001
The in vitro data suggest that Lec35p controls an aspect of MPD orientation in the endoplasmic reticulum membrane that is crucial for its activity as a donor substrate and has an essential role for all known classes of monosaccharide-P-dolichol-dependent reactions in mammals.
Molecular Characterization of the viaB Locus Encoding the Biosynthetic Machinery for Vi Capsule Formation in Salmonella Typhi
- Michael Wetter, D. Goulding, M. Wacker
- BiologyPLoS ONE
- 21 September 2012
The Vi capsular polysaccharide of Salmonella enterica serovar Typhi, the cause of human typhoid, is important for infectivity and virulence and the viaB locus is cloned from S. Typhi and transposon insertion mutants of individual viaB genes were characterized in Escherichia coli DH5α.
A Novel Epimerase That Converts GlcNAc-P-P-undecaprenol to GalNAc-P-P-undecaprenol in Escherichia coli O157*
- J. Rush, C. Alaimo, Riccardo Robbiani, M. Wacker, C. J. Waechter
- Biology, ChemistryJournal of Biological Chemistry
- 18 November 2009
GalNAc-p-P-Und is synthesized reversibly by a novel GlcNAc/UDP- GalNAc epimerase after the formation of Glc NAc-P/P-und by WecA in E. coli O157.
Congenital disorder of glycosylation due to DPM1 mutations presenting with dystroglycanopathy-type congenital muscular dystrophy.
- Amy C Yang, B. Ng, L. Mehta
- Biology, MedicineMolecular Genetics and Metabolism
- 1 November 2013
...
...