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Evidence That the wzxE Gene of Escherichia coli K-12 Encodes a Protein Involved in the Transbilayer Movement of a Trisaccharide-Lipid Intermediate in the Assembly of Enterobacterial Common Antigen*
Results support the conclusion that thewzxE gene encodes a membrane protein involved in the transbilayer movement of lipid III in E. coli. Expand
The ins(ide) and out(side) of dolichyl phosphate biosynthesis and recycling in the endoplasmic reticulum.
The precursor oligosaccharide donor for protein N-glycosylation in eukaryotes, Glc3Man9GlcNAc(2)-P-P-dolichol, is synthesized in two stages on both leaflets of the rough endoplasmic reticulum (ER).Expand
Biosynthesis of Mycobacterial Lipoarabinomannan*
A novel form of cell-free incubation involving in vitro and in situ labeling with GDP-[14C]Man of the polyprenyl-P-mannoses and the simpler PIMs of mycobacterial membranes demonstrated the synthesis of a novel α(1→6)-linked linear form of LM at the expense of the C35/C50-P -Man. Expand
Nogo‐B receptor is necessary for cellular dolichol biosynthesis and protein N‐glycosylation
Identification of Nogo‐B receptor (NgBR) as an essential component of the cis‐IPTase machinery yields insights into the regulation of dolichol biosynthesis. Expand
Novel salvage pathway utilizing farnesol and geranylgeraniol for protein isoprenylation.
Deficiency of UDP‐GlcNAc:Dolichol Phosphate N‐Acetylglucosamine‐1 Phosphate Transferase (DPAGT1) Causes a Novel Congenital Disorder of Glycosylation Type Ij
A novel CDG type, CDG‐Ij, is identified, resulting from deficiency in UDP‐GlcNAc: dolichol phosphate N‐acetyl‐glucosamine‐1 phosphate transferase (GPT) activity encoded by DPAGT1, which is concluded to be responsible for the CDG symptoms in this patient. Expand
Requirement of the Lec35 gene for all known classes of monosaccharide-P-dolichol-dependent glycosyltransferase reactions in mammals.
The in vitro data suggest that Lec35p controls an aspect of MPD orientation in the endoplasmic reticulum membrane that is crucial for its activity as a donor substrate and has an essential role for all known classes of monosaccharide-P-dolichol-dependent reactions in mammals. Expand
A Novel Epimerase That Converts GlcNAc-P-P-undecaprenol to GalNAc-P-P-undecaprenol in Escherichia coli O157*
GalNAc-p-P-Und is synthesized reversibly by a novel GlcNAc/UDP- GalNAc epimerase after the formation of Glc NAc-P/P-und by WecA in E. coli O157. Expand
Molecular Characterization of the viaB Locus Encoding the Biosynthetic Machinery for Vi Capsule Formation in Salmonella Typhi
The Vi capsular polysaccharide of Salmonella enterica serovar Typhi, the cause of human typhoid, is important for infectivity and virulence and the viaB locus is cloned from S. Typhi and transposon insertion mutants of individual viaB genes were characterized in Escherichia coli DH5α. Expand
Farnesol is utilized for isoprenoid biosynthesis in plant cells via farnesyl pyrophosphate formed by successive monophosphorylation reactions.
These results document the presence of at least two CTP-mediated kinases that provide a mechanism for the utilization of F-OH and GG-OH for the biosynthesis of isoprenoid lipids and protein isopranylation. Expand