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Expression of the chlI, chlD, and chlH Genes from the Cyanobacterium Synechocystis PCC6803 in Escherichia coli and Demonstration That the Three Cognate Proteins Are Required for
Magnesium-protoporphyrin chelatase catalyzes the first step unique to chlorophyll synthesis: the insertion of Mg2+ into protoporphyrin IX. Genes from Synechocystis sp. PCC6803 with homology to theExpand
Transfer of Genes Coding for Apoproteins of Reaction Centre and Light-harvesting LH1 Complexes to Rhodobacter sphaeroides
Summary: Several methods of introducing mobilizable cloning vectors by conjugation into the photosynthetic bacterium Rhodobacter sphaeroides have been examined. The efficiency of transfer wasExpand
A Putative Mg Chelatase Subunit from Arabidopsis thaliana cv C24 (Sequence and Transcript Analysis of the Gene, Import of the Protein into Chloroplasts, and in Situ Localization of the Transcript and
We have isolated and sequenced a cDNA from Arabidopsis thaliana cv C24 that encodes a putative Mg chelatase subunit. The deduced amino acid sequence shows a very high level of identity to a geneExpand
Structural and biochemical characterization of Gun4 suggests a mechanism for its role in chlorophyll biosynthesis.
Gun4 has been implicated in a developmental signaling pathway between the chloroplast and the nucleus involving magnesium protoporphyrin IX (MgP(IX)), the first dedicated intermediate in theExpand
Three separate proteins constitute the magnesium chelatase of Rhodobacter sphaeroides.
The insertion of magnesium into protoporphyrin IX is the first step unique to chlorophyll production and is catalyzed by magnesium chelatase. The Rhodobacter sphaeroides genes, bchI and bchDExpand
A Cyanobacterial Chlorophyll Synthase-HliD Complex Associates with the Ycf39 Protein and the YidC/Alb3 Insertase[W][OPEN]
Chlorophyll synthase attaches geranylgeraniol to the chlorophyll macrocycle and was used as bait to retrieve an enzymatically active complex comprising the synthase, the high-light-inducible proteinExpand
Magnesium-dependent ATPase Activity and Cooperativity of Magnesium Chelatase from Synechocystis sp. PCC6803*
The first committed step in chlorophyll biosynthesis is catalyzed by magnesium chelatase, a complex enzyme with at least three substrates, cooperative Mg2+ activation, and free energy couplingExpand
Conserved Chloroplast Open-reading Frame ycf54 Is Required for Activity of the Magnesium Protoporphyrin Monomethylester Oxidative Cyclase in Synechocystis PCC 6803
Background: The cyclase step in chlorophyll biosynthesis remains uncharacterized. Results: Ycf54 forms a complex with other oxidative cyclase components, and a ycf54 mutant accumulates the cyclaseExpand
Magnesium-protoporphyrin chelatase of Rhodobacter sphaeroides: reconstitution of activity by combining the products of the bchH, -I, and -D genes expressed in Escherichia coli.
Magnesium-protoporphyrin chelatase lies at the branch point of the heme and (bacterio)chlorophyll biosynthetic pathways. In this work, the photosynthetic bacterium Rhodobacter sphaeroides has beenExpand
Characterization of the binding of deuteroporphyrin IX to the magnesium chelatase H subunit and spectroscopic properties of the complex.
Magnesium protoporphyrin chelatase catalyzes the insertion of a Mg(2+) ion into protoporphyrin IX, which can be considered as the first committed step of (bacterio)chlorophyll synthesis. In theExpand