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Systemic RNAi in C. elegans Requires the Putative Transmembrane Protein SID-1
Double-stranded RNA-mediated gene interference (RNAi) inCaenorhabditis elegans systemically inhibits gene expression throughout the organism. To investigate how gene-specific silencing information isExpand
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Composition and dynamics of the Caenorhabditis elegans early embryonic transcriptome
Temporal profiles of transcript abundance during embryonic development were obtained by whole-genome expression analysis from precisely staged C. elegans embryos. The result is a highly resolved timeExpand
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Transport of dsRNA into Cells by the Transmembrane Protein SID-1
RNA interference (RNAi) spreads systemically in plants and nematodes to silence gene expression distant from the site of initiation. We previously identified a gene, sid-1, essential for systemic butExpand
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Caenorhabditis elegans SID-2 is required for environmental RNA interference
In plants and in the nematode Caenorhabditis elegans, an RNAi signal can trigger gene silencing in cells distant from the site where silencing is initiated. In plants, this signal is known to be aExpand
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Quantitative analysis of mRNA amplification by in vitro transcription.
Effective transcript profiling in animal systems requires isolation of homogenous tissue or cells followed by faithful mRNA amplification. Linear amplification based on cDNA synthesis and in vitroExpand
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An antiviral role for the RNA interference machinery in Caenorhabditis elegans.
RNA interference (RNAi) is a sequence-specific gene-silencing mechanism triggered by exogenous dsRNA. In plants an RNAi-like mechanism defends against viruses, but the hypothesis that animals possessExpand
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Spatial and Temporal Controls Target pal-1 Blastomere-Specification Activity to a Single Blastomere Lineage in C. elegans Embryos
The early asymmetric cleavages of Caenorhabditis elegans embryos produce blastomeres with distinct developmental potentials. Here, we show that the caudal-like homeodomain protein PAL-1 is requiredExpand
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Genomic analysis of gene expression in C. elegans.
Until now, genome-wide transcriptional profiling has been limited to single-cell organisms. The nematode Caenorhabditis elegans is a well-characterized metazoan in which the expression of all genesExpand
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Export of RNA silencing from C. elegans tissues does not require the RNA channel SID-1
Double-stranded RNA (dsRNA) triggers RNA interference (RNAi) to silence genes of matching sequence. In some animals this experimentally induced silencing is transported between cells, and studies inExpand
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The homeodomain protein PAL-1 specifies a lineage-specific regulatory network in the C. elegans embryo
Maternal and zygotic activities of the homeodomain protein PAL-1 specify the identity and maintain the development of the multipotent C blastomere lineage in the C. elegans embryo. To identify PAL-1Expand
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