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Bile salt–induced intermolecular disulfide bond formation activates Vibrio cholerae virulence
A mechanism for direct activation of the V. cholerae virulence cascade by a host signal molecule is demonstrated and a model in which the other cysteine in the periplasmic domain forms an inhibitory intramolecular disulfide bond with C207 that must be isomerized to form the active C207–C207 intermolecular bond is proposed. Expand
The marinopyrroles, antibiotics of an unprecedented structure class from a marine Streptomyces sp.
Cultivation of an obligate marine Streptomyces strain has furnished the marinopyrroles A and B, densely halogenated, axially chiral metabolites that contain an uncommon bispyrrole structure that possess potent antibiotic activities against methicillin-resistant Staphylococcus aureus. Expand
Antibacterials from the sea.
A concerted effort to discover new antibacterials from marine sources has the potential to contribute significantly to the treatment of the ever increasing drug-resistant infectious diseases. Expand
Marinopyrrole A target elucidation by acyl dye transfer.
The combination of cell, protein, and amino acid analysis arose from a single sample of material, thereby, suggesting a means to streamline and reduce material requirements involved in mode of action studies. Expand
The role of inter-species interactions in Salinispora specialized metabolism.
The results show that antibiotic activities and the concentration of some small molecules increase in the presence of co-occurring bacterial strains relative to monocultures and some increases were correlated with the production of specific compounds by S. tropica. Expand
The ammosamides: structures of cell cycle modulators from a marine-derived Streptomyces species.
Marine-derived actinomycete bacteria are emerging as a valuable resource for bioactive natural products encompassing a variety of unique structural classes.[1] In our hands, early detection of cellExpand
Total synthesis of (-)-heptemerone B and (-)-guanacastepene E.
A concise, stereoselective, and convergent total synthesis of the unnatural enantiomer of the neodolastane diterpenoid heptemerone B has been completed and the absolute stereochemistry of (-)-heptemer one B was established as 5-(S), the same as (-)-guanacastepene E. Expand
Function-oriented biosynthesis of beta-lactone proteasome inhibitors in Salinispora tropica.
One of the engineered compounds is equipotent to salinosporamide A in inhibition of the chymotrypsin-like activity of the proteasome yet exhibits superior activity in the cell-based HCT-116 assay. Expand
Chlorizidine, a cytotoxic 5H-pyrrolo[2,1-a]isoindol-5-one-containing alkaloid from a marine Streptomyces sp.
X-ray crystallographic analysis revealed that the metabolite is composed of a chlorinated 2,3-dihydropyrrolizine ring attached to achlorinated 5H-pyrrolo[2,1-a]isoindol-5-one moiety, which is essential to the molecule's cytotoxicity against HCT-116 human colon cancer cells. Expand