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Dehalobacter restrictus gen. nov. and sp. nov., a strictly anaerobic bacterium that reductively dechlorinates tetra- and trichloroethene in an anaerobic respiration
It is proposed to affiliate the isolate to a new genus, Dehalobacter, with PER-K23 as the type strain of the new species De Halobacter restrictus, based on the cytological, physiological, and phylogenetic characterization of the isolate.
Reductive dechlorination in the energy metabolism of anaerobic bacteria
Within the last few decades, several anaerobic bacteria have been isolated which are able to reductively dechlorinate chlorinated aliphatic and aromatic compounds at catabolic rates. For some of
A highly purified enrichment culture couples the reductive dechlorination of tetrachloroethene to growth
H2 consumption was paralleled by chloride production, PCE degradation, cis-1,2-DCE formation, and growth of PER-K23, and Electron balances showed that all electrons derived from H2 or formate consumption were recovered in dechlorination products and biomass.
Biochemistry of Microbial Degradation of Hexachlorocyclohexane and Prospects for Bioremediation
It is important to develop a better understanding of the biochemistries of the LinA and LinB variants and to use that knowledge to build better variants, because field trials of some bioremediation strategies based on the Lin pathway have yielded promising results but would not yet achieve economic levels of remediation.
Characterization of the Corrinoid Iron-Sulfur Protein Tetrachloroethene Reductive Dehalogenase of Dehalobacter restrictus
The membrane-bound tetrachloroethene reductive dehalogenase (PCE-RDase) (PceA), the terminal component of the respiratory chain of Dehalobacter restrictus, was purified 25-fold to apparent electrophoretic homogeneity and formed a coherent group of reductiveDehalogenases with almost 100% sequence identity.
Vertical Distribution of Methanogens in the Anoxic Sediment of Rotsee (Switzerland)
ABSTRACT Anoxic sediments from Rotsee (Switzerland) were analyzed for the presence and diversity of methanogens by using molecular tools and for methanogenic activity by using radiotracer techniques,
Complete Reduction of TNT and Other (Poly)nitroaromatic Compounds under Iron-Reducing Subsurface Conditions
Contamination of soils and aquifers with (poly)nitroaromatic compounds ((P)NACs) is a widespread problem. This work demonstrates that (P)NACs such as the explosive 2,4,6-trinitrotoluene (TNT) can be
Development of a real-time PCR method for quantification of the three genera Dehalobacter, Dehalococcoides, and Desulfitobacterium in microbial communities.
Determination of the fractional numbers in DNA mixtures of known composition showed the accuracy of the standard curves developed, and indicated that the low amplification efficiency of certain standard curves seemed to be mainly a problem of the plasmid DNA used and not of the 16S rRNA gene of the target genera.
Localization and Solubilization of the Iron(III) Reductase of Geobacter sulfurreducens
It can be hypothesized that the iron(III) reductase of G. sulfurreducens is a peripheral outer membrane protein that might contain ac-type cytochrome.
Enrichment and properties of an anaerobic mixed culture reductively dechlorinating 1,2,3-trichlorobenzene to 1,3-dichlorobenzene
Enrichment cultures originated from percolation columns filled with Rhine River sediment in which dechlorination of TCBs and dichlorobenzenes (DCBs) occurred and Methanogens and acetogens were the major substrate-competing (H2-CO2) microorganisms in the two-liquid-phase system.