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Real-time RT-PCR: considerations for efficient and sensitive assay design.
The effect of amplicon secondary structure on reaction efficiency and its importance for primer design is demonstrated and MMLV and AMV enzyme systems using a variety of RT priming methods are compared and the problem of primer-dimer formation associated with RT enzymes is described.
Prevalence of Hemotropic Mycoplasmas in Healthy and Unhealthy Cats and Dogs in Spain
The aims of the present study were to determine the prevalence of hemoplasmas in cats and dogs from the Barcelona area of Spain with the use of species-specific quantitative polymerase chain reaction
Phylogenetic Analysis of Hemoplasma Species: an International Study
Nearly complete 16S rRNA gene sequences for feline and canine hemoplasma isolates from Europe, Australia, Africa, and Asia showed almost 100% identity to those previously reported for United States isolates, and phylogenetic analysis showed that the hemoplasmas are most closely related to the members of the Mycoplasma pneumoniae group.
Use of a PCR assay to assess the prevalence and risk factors for Mycoplasma haemofelis and ‘Candidatus Mycoplasma haemominutum’ in cats in the United Kingdom
Logistic regression analysis indicated that older male cats were significantly more likely to be infected with ‘Candidatus M haemominutum’, but there was no significant association between it and any of the haematological variables measured.
Potential contamination of beef carcases with brain tissue at slaughter
EXPERIMENTAL strain typing of variant Creutzfeldt-Jakob disease (VCJD) has shown that the transmissible agent responsible for this disorder is identical to that in bovine spongiform encephalopathy
Antibody and cytokine responses in kittens during the development of feline infectious peritonitis (FIP)
Two recombinant FIPV spike proteins were assessed for their immunogenic properties in 8-week-old kittens, which were then challenged intranasally with FipV 79-1146, and failed to alter the course of disease compared to controls.
Cytokine mRNA Expression in Mucosal Biopsies from German Shepherd Dogs with Small Intestinal Enteropathies
Altered alterations in cytokine mRNA expression suggest heightened immune responses within the duodenal mucosa in GSDs with either SIBO or IBD.
Amino acid changes in the spike protein of feline coronavirus correlate with systemic spread of virus from the intestine and not with feline infectious peritonitis
The methionine to leucine substitution at position 1058 in the FCoV spike protein is indicative of systemic spread of F CoV from the intestine, rather than a virus with the potential to cause FIP.
Development and application of multiple internal reference (housekeeper) gene assays for accurate normalisation of canine gene expression studies.
There are no universal control genes for gene expression studies in canine tissues, and distinct internal control genes were shown to be most appropriate for use in full-thickness versus superficial mucosal biopsies of the duodenum.
Factors associated with upper respiratory tract disease caused by feline herpesvirus, feline calicivirus, Chlamydophila felis and Bordetella bronchiseptica in cats: experience from 218 European
There was evidence that FHV, FCV and B bronchiseptica played a role in URTD, and the risk factors associated with the disease were less than excellent hygiene, contact with dogs with URTd, and larger numbers of cats in the cattery or household.