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The enzymatic basis for pesticide bioremediation
TLDR
This review describes examples of enzymes possessing the major activities employed in the bioremediation of pesticide residues, and some of the strategies by which they are employed.
Amplification of DNA from preserved specimens shows blowflies were preadapted for the rapid evolution of insecticide resistance.
TLDR
The early outbreak of resistance in this species can be explained by the preexistence of mutant alleles encoding malathion resistance, and the pinned specimen analysis shows much higher genetic diversity at the locus before organophosphate use, suggesting that the subsequent sweep of diazinon resistance in Australasia has compromised the scope for the loci to respond further to the ongoing challenge of the insecticides.
Kelp Fly Virus: a Novel Group of Insect Picorna-Like Viruses as Defined by Genome Sequence Analysis and a Distinctive Virion Structure
TLDR
The complete genomic sequence of kelp fly virus (KFV) has been determined and analyses of its genomic and structural organization and phylogeny show that it belongs to a hitherto undescribed group within the picorna-like virus superfamily.
An unexpected vestigial protein complex reveals the evolutionary origins of an s-triazine catabolic enzyme
TLDR
It is proposed that AtzEG was likely recruited into the cyanuric acid–mineralizing pathway from an ancestral glutamine transamidosome that required protein–protein interactions to enforce the exclusion of solvent from the transamidation reaction.
Bacterial biodegradation of neonicotinoid pesticides in soil and water systems.
TLDR
This review focuses on the biodegradation of neonicotinoid-mineralising bacterial strains in soil and water systems by the bacterial community and indicates that enhanced biotransformation of these pesticides can be accomplished by mixed microbial populations under optimised environmental conditions.
Production of D-amino acids from D,L-5-substituted hydantoins by an Agrobacterium tumefaciens strain and isolation of a mutant with inducer-independent expression of hydantoin-hydrolysing activity
TLDR
A mutant strain of Agrobacterium tumefaciens RU-OR was isolated, which expressed both the hydantoinase and N-carbamylamino acid amidohydrolase enzymes in the absence of inducer.
Structural and biochemical characterization of the biuret hydrolase (BiuH) from the cyanuric acid catabolism pathway of Rhizobium leguminasorum bv. viciae 3841
TLDR
The structure of the inactive Cys175Ser BiuH variant with substrate bound in the active site revealed that an active site cysteine (Cys175), aspartic acid (Asp36) and lysine (Lys142) form a catalytic triad, which is consistent with biochemical studies ofBiuH variants.
Elucidation and manipulation of the Hydantoin-Hydrolysing Enzyme System of Agrobacterium tumefaciens RU-OR for the Biocatalytic production of D-amino acids
TLDR
Characterisation of the hydantoin-hydrolysing enzyme system of RU-OR revealed novel bioc atalytic properties, and potential for the application of this strain for the biocatalytic production of D-amino acids.
Engineered enzymes that retain and regenerate their cofactors enable continuous-flow biocatalysis
TLDR
This work reports immobilized enzymes with a tethered cofactor that is channelled between a recycling and a catalysis module facilitating total turnover numbers of NAD+ and ATP exceeding 10,000 in biocatalytic flow systems.
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